| SYSNO ASEP | 0023757
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| Druh ASEP | J - Článek v odborném periodiku
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| Zařazení RIV | J - Článek v odborném periodiku
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| Poddruh J | Ostatní články
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| Název | Downregulation of the nuclear-encoded subunits of the complexes III and IV disrupts their respective complexes but not complex I in procyclic Trypanosoma brucei
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| Překlad názvu | Eliminace jaderně kódovaných podjednotek komplexů III a IV způsobuje rozpad těchto komplexů ale ne komplexu I u Trypanosoma brucei
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| Tvůrce(i) | Horváth, A. (SK)
Horáková, Eva (PAU-O)
Dunajčíková, P. (SK)
Verner, Zdeněk (PAU-O)
Pravdová, E. (SK)
Šlapetová, Iveta (PAU-O)
Cuninková, Ľ. (SK)
Lukeš, Julius (PAU-O)
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| Zdroj.dok. | Molecular Microbiology
- ISSN 0950-382X
Roč. 58, č. 1 (2005), s. 116-130
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| Poč.str. | 15 s.
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| Jazyk dok. | eng - angličtina
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| Země vyd. | GB - Velká Británie
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| Klíč. slova | respiratory complex ; Trypanosoma ; RNA interference
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| Vědní obor RIV | EB - Genetika a molekulární biologie
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| CEP | IAA5022302 GA AV ČR - Akademie věd
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| CEZ | AV0Z60220518 - PAU-O, BC-A (2005-2011)
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| Anotace | The function, stability and mutual interactions of selected nuclear-encoded subunits of respiratory complexes III and IV were studied in the Trypanosoma brucei procyclics using RNA interference (RNAi). Western analysis of mitochondrial lysates using specific antibodies confirmed complete elimination of the targeted proteins 4 to 6 days post-induction. As revealed by two-dimensional blue native/SDS PAGE electrophoresis, silencing of a single subunit resulted in the disruption of the respective complex, while the other complex remained unaffected. Membrane potential was reproducibly decreased in the knock-downs and the activities of complex III and/or IV, but not complex I, were drastically reduced, as measured by activity assays and histochemical staining. The apparent absence in T. brucei procyclics of a supercomplex composed of complexes I and III may represent an ancestral state of the respiratory chain.
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| Pracoviště | Parazitologický ústav (do r. 2005)
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| Kontakt | Kateřina Homrová,katerina.homrova@paru.cas.cz , Tel.: 387 775 406
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| Rok sběru | 2006
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