Počet záznamů: 1
Microtubule-associated proteins MAP7 and MAP7D1 promote DNA double-strand break repair in the G1 cell cycle phase
- 1.
SYSNO ASEP 0582795 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Microtubule-associated proteins MAP7 and MAP7D1 promote DNA double-strand break repair in the G1 cell cycle phase Tvůrce(i) Dullovi, A. (GB)
Ozgencil, M. (GB)
Rajvee, V. (GB)
Tse, W. Y. (GB)
Cutillas, P. R. (GB)
Martin, S. A. (GB)
Hořejší, Zuzana (UOCHB-X)Číslo článku 106107 Zdroj.dok. iScience. - : Cell Press
Roč. 26, č. 3 (2023)Poč.str. 21 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova DNA damage ; microtubules ; microtubule-associated proteins Obor OECD Biochemistry and molecular biology Způsob publikování Open access Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000993587500001 EID SCOPUS 85148005797 DOI 10.1016/j.isci.2023.106107 Anotace The DNA-damage response is a complex signaling network that guards genomic integrity. The microtubule cytoskeleton is involved in the repair of DNA doublestrand breaks, however, little is known about which cytoskeleton-related pro-teins are involved in DNA repair and how. Using quantitative proteomics, we discovered that microtubule associated proteins MAP7 and MAP7D1 interact with several DNA repair proteins including DNA double-strand break repair pro-teins RAD50, BRCA1 and 53BP1. We observed that downregulation of MAP7 and MAP7D1 leads to increased phosphorylation of p53 after girradiation. More-over, we determined that the downregulation of MAP7D1 leads to a strong G1 arrest and that the downregulation of MAP7 and MAP7D1 in G1 arrested cells negatively affects DNA repair, recruitment of RAD50 to chromatin and localiza-tion of 53BP1 to the sites of damage. These findings describe for the first time a novel function of MAP7 and MAP7D1 in cell cycle regulation and repair of DNA double-strand breaks. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2024 Elektronická adresa https://doi.org/10.1016/j.isci.2023.106107
Počet záznamů: 1