Počet záznamů: 1
Exploring the crop epigenome: a comparison of DNA methylation profiling techniques
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SYSNO ASEP 0575584 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Exploring the crop epigenome: a comparison of DNA methylation profiling techniques Tvůrce(i) Agius, D. R. (MT)
Kapazoglou, A. (GR)
Avramidou, E. (GR)
Baránek, M. (CZ)
Carneros, E. (ES)
Caro, E. (ES)
Castiglione, S. (IT)
Cicatelli, A. (IT)
Radanovic, A. (RS)
Ebejer, J. P. (MT)
Gackowski, D. (PL)
Guarino, F. (IT)
Gulyás, Z. (HU)
Hidvégi, N. (HU)
Hoenicka, H. (DE)
Inácio, V. (PT)
Johannes, F. (DE)
Karalija, E. (BA)
Lieberman-Lazarovich, M. (IL)
Martinelli, F. (IT)
Maury, S. (FR)
Mladenov, V. (RS)
Morais-Cecílio, L. (PT)
Pečinka, Aleš (UEB-Q) ORCID, RID
Tani, E. (GR)
Testillano, P. S. (ES)
Todorov, D. (BG)
Valledor, L. (ES)
Vassileva, V. (BG)Celkový počet autorů 29 Číslo článku 1181039 Zdroj.dok. Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 14, MAY 30 (2023)Poč.str. 24 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova bisulfite sequencing ; crop epigenome ; DNA methylation modulation ; DNA methylation profiling ; immunological techniques ; mass spectrometry ; next-generation sequencing Obor OECD Biochemistry and molecular biology Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 001007263700001 EID SCOPUS 85161895018 DOI 10.3389/fpls.2023.1181039 Anotace Epigenetic modifications play a vital role in the preservation of genome integrity and in the regulation of gene expression. DNA methylation, one of the key mechanisms of epigenetic control, impacts growth, development, stress response and adaptability of all organisms, including plants. The detection of DNA methylation marks is crucial for understanding the mechanisms underlying these processes and for developing strategies to improve productivity and stress resistance of crop plants. There are different methods for detecting plant DNA methylation, such as bisulfite sequencing, methylation-sensitive amplified polymorphism, genome-wide DNA methylation analysis, methylated DNA immunoprecipitation sequencing, reduced representation bisulfite sequencing, MS and immuno-based techniques. These profiling approaches vary in many aspects, including DNA input, resolution, genomic region coverage, and bioinformatics analysis. Selecting an appropriate methylation screening approach requires an understanding of all these techniques. This review provides an overview of DNA methylation profiling methods in crop plants, along with comparisons of the efficacy of these techniques between model and crop plants. The strengths and limitations of each methodological approach are outlined, and the importance of considering both technical and biological factors are highlighted. Additionally, methods for modulating DNA methylation in model and crop species are presented. Overall, this review will assist scientists in making informed decisions when selecting an appropriate DNA methylation profiling method. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2024 Elektronická adresa https://doi.org/10.3389/fpls.2023.1181039
Počet záznamů: 1