Počet záznamů: 1
Determining the Functional Oligomeric State of Membrane- Associated Protein Oligomers Forming Membrane Pores on Giant Lipid Vesicles
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SYSNO ASEP 0572771 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Determining the Functional Oligomeric State of Membrane- Associated Protein Oligomers Forming Membrane Pores on Giant Lipid Vesicles Tvůrce(i) Singh, Vandana (UFCH-W)
Macharová, Sabína (UFCH-W)
Riegerová, Petra (UFCH-W) ORCID
Steringer, J. P. (DE)
Mueller, H. (DE)
Lolicato, F. (FI)
Nickel, W. (DE)
Hof, Martin (UFCH-W) RID, ORCID
Šachl, Radek (UFCH-W) RID, ORCIDZdroj.dok. Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 95, č. 23 (2023), s. 8807-8815Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Cell culture ; Fibroblasts ; Membranes Vědní obor RIV CF - Fyzikální chemie a teoretická chemie Obor OECD Physical chemistry CEP GC20-01401J GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UFCH-W - RVO:61388955 UT WOS 000985563200001 EID SCOPUS 85159619887 DOI 10.1021/acs.analchem.2c05692 Anotace Several peripheral membrane proteins are known to form membrane pores through multimerization. In many cases, in biochemical reconstitution experiments, a complex distribution of oligomeric states has been observed that may, in part, be irrelevant to their physiological functions. This phenomenon makes it difficult to identify the functional oligomeric states of membrane lipid interacting proteins, for example, during the formation of transient membrane pores. Using fibroblast growth factor 2 (FGF2) as an example, we present a methodology applicable to giant lipid vesicles by which functional oligomers can be distinguished from nonspecifically aggregated proteins without functionality. Two distinct populations of fibroblast growth factor 2 were identified with (i) dimers to hexamers and (ii) a broad population of higher oligomeric states of membrane associated FGF2 oligomers significantly distorting the original unfiltered histogram of all detectable oligomeric species of FGF2. The presented statistical approach is relevant for various techniques for characterizing membrane-dependent protein oligomerization. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2024 Elektronická adresa https://hdl.handle.net/11104/0343342
Počet záznamů: 1