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Transcriptomic hallmarks of in vitro TiOinf2/inf nanotubes toxicity in Chlamydomonas reinhardtii
- 1.0571826 - ÚEB 2024 RIV NL eng J - Článek v odborném periodiku
Dobešová, M. - Koláčková, M. - Pencik, O. - Cápal, Petr - Chaloupsky, P. - Švec, P. - Ridošková, A. - Motola, M. - Cicmancova, V. - Sopha, H. - Macák, J. M. - Richtera, L. - Adam, V. - Húska, D.
Transcriptomic hallmarks of in vitro TiOinf2/inf nanotubes toxicity in Chlamydomonas reinhardtii.
Aquatic Toxicology. Roč. 256, MAR (2023), č. článku 106419. ISSN 0166-445X. E-ISSN 1879-1514
Institucionální podpora: RVO:61389030
Klíčová slova: Chlamydomonas * Nanotubes * RNA-seq * TiO 2 * Toxicity
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 4.5, rok: 2022
Způsob publikování: Open access
https://doi.org/10.1016/j.aquatox.2023.106419
Recently, more accessible transcriptomic approaches have provided a new and deeper understanding of environmental toxicity. The present study focuses on the transcriptomic profiles of green microalgae Chlamydomonas reinhardtii exposed to new industrially promising material, TiO2 nanotubes (NTs), as an example of a widely used one-dimensional nanomaterial. The first algal in vitro assay included 2.5 and 7.5 mg/L TiO2 NTs, resulting in a dose-dependent negative effect on biological endpoints. At a working concentration of 7.5 mg/L, RNA-sequencing showed a mainly negative effect on the cells. In summary, the results indicated metabolic disruption, such as ATP loss, damage to mitochondria and chloroplasts, loss of solutes due to permeated membranes, and cell wall damage. Moreover, apoptosis-induced transcripts were detected. Interestingly, reactivation of transposons was observed. In signalling and transcription pathways, including chromatin remodelling and locking, the annotated genes were downregulated.
Trvalý link: https://hdl.handle.net/11104/0342736
Název souboru Staženo Velikost Komentář Verze Přístup 2023_Dobesova_AQUATIC TOXICOLOGY_106419.pdf 4 8.6 MB Jiná povolen
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