Počet záznamů: 1
Diamond Surfaces with Clickable Antifouling Polymer Coating for Microarray-Based Biosensing
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SYSNO ASEP 0562670 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Diamond Surfaces with Clickable Antifouling Polymer Coating for Microarray-Based Biosensing Tvůrce(i) Kumar, R. (DE)
Yang, B. (DE)
Bartoň, Jan (UOCHB-X) RID, ORCID
Štejfová, Miroslava (UOCHB-X) ORCID
Schaefer, A. (DE)
König, M. (DE)
Knittel, P. (DE)
Cígler, Petr (UOCHB-X) RID, ORCID
Hirtz, M. (DE)Číslo článku 2201453 Zdroj.dok. Advanced Materials Interfaces. - : Wiley - ISSN 2196-7350
Roč. 9, č. 33 (2022)Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova antifouling polymer coating ; copper catalyzed alkyne-azide cycloaddition ; diamond surfaces ; fluorescence imaging ; microchannel cantilever spotting ; selective protein binding Obor OECD Materials engineering CEP EF16_026/0008382 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000865567900001 EID SCOPUS 85139453225 DOI 10.1002/admi.202201453 Anotace Diamond enables the construction of various (bio)sensors, including those with quantum-based detection. However, bare diamond interfaces are susceptible to unspecific adhesion of proteins and other macromolecules from biological media or complex samples. This impairs selectivity in biosensing, leads to low signal-to-noise ratio in fluorescence-based applications, and introduces the need for blocking steps in incubation protocols. Here, a stable, protein-repellent, and clickable reactive polymer coating is introduced, abolishing unspecific protein adhesion while concurrently enabling covalent immobilization of functional compounds as recognition elements. The polymer coating has two segments, an antifouling poly(N-(2-hydroxypropyl) methacrylamide) and an alkyne-terminated poly(propargyl methacrylamide) providing the click functionality. The antifouling properties and click-reactivity of the polymers are demonstrated by selective protein binding assays on micropatterns written by microchannel cantilever spotting (mu CS). The assays demonstrated the successful functionalization of both diamond and glass surfaces and the excellent antifouling properties of the polymer coating. The coating procedure is compatible with oxidized diamond surfaces thus well-suitable for diamond-based quantum technology. The results can directly impact applications of diamond materials in optically detected quantum sensing or fluorescence sensing in general. The polymer functionalization can also be used for any case where highly specific interaction with low fouling is desired. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2023 Elektronická adresa https://doi.org/10.1002/admi.202201453
Počet záznamů: 1