The Complete Genome Sequence and Structure of the Oleaginous Rhodococcus opacus Strain PD630 Through Nanopore Technology

0556828 - MBÚ 2023 RIV CH eng J - Článek v odborném periodiku
Firrincieli, A. - Grigoriev, B. - Dostálová, Hana - Cappelletti, M.
The Complete Genome Sequence and Structure of the Oleaginous Rhodococcus opacus Strain PD630 Through Nanopore Technology.
Frontiers in Bioengineering and Biotechnology. Roč. 9, FEB 17 (2022), č. článku 810571. ISSN 2296-4185. E-ISSN 2296-4185
Institucionální podpora: RVO:61388971
Klíčová slova: stress * Rhodococcus opacus PD630 * nanopore sequencing * oleaginous bacteria * complete genome * Rhodococcus genomics * Rhodococcus opacus plasmids * xenobiotic degradation genes * lipid metabolism genes
Obor OECD: Microbiology
Impakt faktor: 5.7, rok: 2022
Způsob publikování: Open access
https://www.frontiersin.org/articles/10.3389/fbioe.2021.810571/full

The complete genome sequence of oleaginous strain Rhodococcus opacus PD630 from the Leibniz institute DSMZ-German collection of microorganisms and cell cultures (DSM 44 193, Braunschweig, Germany) was obtained by combination of Oxford Nanopore sequence technology with Illumina high quality sequencing data. One chromosome (8.37 Mbp), one linear (pRoPD630_1, 538 909 bp) and two circular (pRoPD630_2, 167 985 bp and pRoPD630_3, 85 051 bp) plasmid sequences were determined and annotated. This work provides the correct R. opacus PD630 genomic framework for the development of genetic engineering strategies to boost the application of this strain as microbial cell factory for lipid production.



Trvalý link: http://hdl.handle.net/11104/0331261