Antiviral Activity of Vacuolar ATPase Blocker Diphyllin against SARS-CoV-2

0555028 - BC 2022 RIV CH eng J - Článek v odborném periodiku
Štefánik, M. - Straková, P. - Haviernik, J. - Miller, A. - Růžek, Daniel - Eyer, Luděk
Antiviral Activity of Vacuolar ATPase Blocker Diphyllin against SARS-CoV-2.
Microorganisms. Roč. 9, č. 3 (2021), č. článku 471. E-ISSN 2076-2607
Institucionální podpora: RVO:60077344
Klíčová slova: inhibitor * glycosides * Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) * vacuolar ATPase blocker * diphyllin * cleistanthin B * helioxanthin * antiviral activity * cytotoxicity
Obor OECD: Microbiology
Impakt faktor: 4.926, rok: 2021
Způsob publikování: Open access
https://www.mdpi.com/2076-2607/9/3/471

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a causative agent of the pandemic coronavirus disease 2019 (COVID-19), which has resulted in over two million deaths worldwide to date. Diphyllin and diphyllinosides are known as natural blockers of cellular vacuolar ATPases, and so can act as inhibitors of the pH-dependent fusion of viral envelopes with host cell endosomal membranes. Such pH-dependent fusion is a critical early step during the SARS-CoV-2 replication cycle. Accordingly, the anti-SARS-CoV-2 profiles and cytotoxicities of diphyllin, diphyllinoside cleistanthin B, and two structurally related compounds, helioxanthin 8-1 and helioxanthin 5-4-2, are evaluated here using in vitro cell-based assay systems. Neither helioxanthin exhibits any obvious anti-SARS-CoV-2 effects in vitro. By contrast diphyllin and cleistanthin B do exhibit anti-SARS-CoV-2 effects in Vero cells, with respective 50% effective concentrations (EC50) values of 1.92 and 6.51 mu M. Diphyllin displays anti-SARS-CoV-2 effect also in colorectal adenocarcinoma (CaCo-2) cells. Moreover, when diphyllin is added at various times post infection, a significant decrease in viral titer is observed in SARS-CoV-2-infected Vero cells, even at high viral multiplicities of infection. Importantly, neither diphyllin nor cleistanthin B are found cytotoxic to Vero cells in concentrations up to 100 mu M. However, the cytotoxic effect of diphyllin is more pronounced in Vero E6 and CaCo-2 cells. Overall, our data demonstrate that diphyllin and diphyllin analogues might be perfected as anti-SARS-CoV-2 agents in future preclinical studies, most especially if nanomedicine approaches may be invoked to optimize functional drug delivery to virus infected cells.
Trvalý link: http://hdl.handle.net/11104/0329646