Počet záznamů: 1
Single Molecule RNA Localization and Translation in the Mammalian Oocyte and Embryo
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SYSNO ASEP 0546076 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Single Molecule RNA Localization and Translation in the Mammalian Oocyte and Embryo Tvůrce(i) Jansová, Denisa (UZFG-Y) ORCID
Aleshkina, Daria (UZFG-Y)
Jindrová, Anna (UZFG-Y) ORCID
Iyyappan, Rajan (UZFG-Y) ORCID
An, Q. (US)
Fan, G. (US)
Šušor, Andrej (UZFG-Y) RID, ORCIDČíslo článku 167166 Zdroj.dok. Journal of Molecular Biology. - : Elsevier - ISSN 0022-2836
Roč. 433, č. 19 (2021)Poč.str. 15 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova mRNA ; translation ; oocyte Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Developmental biology CEP GA18-19395S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000697111000007 EID SCOPUS 85111943954 DOI 10.1016/j.jmb.2021.167166 Anotace During oocyte growth the cell accumulates RNAs to contribute to oocyte and embryo development which progresses with ceased transcription. To investigate the subcellular distribution of specific RNAs and their translation we developed a technique revealing several instances of localized translation with distinctive regulatory implications. We analyzed the localization and expression of candidate non-coding and mRNAs in the mouse oocyte and embryo. Furthermore, we established simultaneous visualization of mRNA and in situ translation events validated with polysomal occupancy. We discovered that translationally dormant and abundant mRNAs CyclinB1 and Mos are localized in the cytoplasm of the fully grown GV oocyte forming cloud-like structures with consequent abundant translation at the center of the MII oocyte. Coupling detection of the localization of specific single mRNA molecules with their translation at the subcellular context is a valuable tool to quantitatively study temporal and spatial translation of specific target mRNAs to understand molecular processes in the developing cell. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S002228362100396X?via%3Dihub
Počet záznamů: 1