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Genomic damage induced in Nicotiana tabacum L. plants by colloidal solution with silver and gold nanoparticles
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SYSNO ASEP 0545280 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Genomic damage induced in Nicotiana tabacum L. plants by colloidal solution with silver and gold nanoparticles Tvůrce(i) Lovecká, P. (CZ)
Macůrková, A. (CZ)
Záruba, K. (CZ)
Hubáček, Tomáš (BC-A) RID
Siegel, J. (CZ)
Valentová, O. (CZ)Číslo článku 1260 Zdroj.dok. Plants. - : MDPI
Roč. 10, č. 6 (2021)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova comet assay ; tobacco ; silver nanoparticles ; gold nanoparticles Vědní obor RIV JJ - Ostatní materiály Obor OECD Nano-materials (production and properties) Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000666150800001 EID SCOPUS 85108142698 DOI 10.3390/plants10061260 Anotace Tobacco seedlings (Nicotiana tabacum L cv. Wisconsin 38) were treated for 24 h with colloidal solution of silver and gold nanoparticles (AgNPs and AuNPs) of different size or cultivated for 8 weeks on soil polluted with these NPs. DNA damage in leaf and roots nuclei was evaluated by the comet assay. AgNPs of the size 22-25 nm at concentrations higher than 50 mg.L-1 significantly increased the tail moments (TM) values in leaf nuclei compared to the negative control. Ag nanoparticles of smaller size 12-15 nm caused a slight increase in tail moment without significant difference from the negative control. The opposite effect of AgNPs was observed on roots. The increasing tail moment was registered for smaller NPs. Similar results were observed for AuNPs at a concentration of 100 mg.L-1. DNA damaging effects after growing tobacco plants for 8 weeks in soil polluted with AgNPs and AuNPs of different size and concentrations were observed. While lower concentrations of both types of particles had no effect on the integrity of DNA, concentration of 30 mg.kg(-1) of AgNPs caused significant DNA damage in leaves of tobacco plants. AuNPs had no effect even at the highest concentration. The content of Ag was determined by ICP-MS in above-ground part of plants (leaves) after 8 weeks of growth in soil with 30 mg.kg(-1). AgNPs and was 2.720 +/- 0.408 µg.g(-1). Long term effect is much less harmful probably due to the plant restoration capability. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/2223-7747/10/6/1260
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