Micellar electrokinetic chromatography as a powerful analytical tool for research on prebiotic chemistry

0543635 - ÚFCH JH 2022 RIV US eng J - Článek v odborném periodiku
Zemánková, K. - Nejdl, L. - Bezděková, J. - Vodová, M. - Petera, Lukáš - Pastorek, Adam - Civiš, Svatopluk - Kubelík, Petr - Ferus, Martin - Adam, V. - Vaculovičová, M.
Micellar electrokinetic chromatography as a powerful analytical tool for research on prebiotic chemistry.
Microchemical Journal. Roč. 167, AUG 2021 (2021), č. článku 106022. ISSN 0026-265X. E-ISSN 1095-9149
Grant CEP: GA ČR GA19-03314S; GA MŠMT EF16_019/0000778
Institucionální podpora: RVO:61388955
Klíčová slova: high-energy chemistry * capillary-electrophoresis * rich atmospheres * hydrogen-cyanide * formamide * origin * purine * bases * nucleobases * adenine * Capillary electrophoresis * Purine * Pyrimidine * Nucleobases * Origin of life * Molecularly imprinted polymers
Obor OECD: Physical chemistry
Impakt faktor: 5.304, rok: 2021
Způsob publikování: Omezený přístup

Capillary electromigration techniques have proven their capabilities in detection of variety of analytes from inorganic ions and small organic molecules through bio(macro)molecules to large analytes such as cells or nano/ micro particles. Also broad range of potential applications includes food and environmental analysis, biomedical and pharmaceutical investigations or even diagnostics.
In this work, it was demonstrated that capillary micellar electrokinetic chromatography is an excellent tool
extremely helpful in investigations focused on prebiotic synthesis of molecules essential for formation of life on
early Earth – nucleobases. In particular, rapid separation of nucleobases (< 2 min) was achieved in 40 mM borate
buffer separation electrolyte containing 60 mM sodium dodecyl sulfate as an additive. This approach enabled
detection of nucleobases formed in thermolysed formamide under conditions simulating the environment
occurring on early Earth. Moreover, polydopamine-based molecularly imprinted polymers specific to thymine
and uracil improved detection of these low abundant products.
Trvalý link: http://hdl.handle.net/11104/0320824