Structural basis of RNA recognition by the SARS-CoV-2 nucleocapsid phosphoprotein

0536797 - ÚOCHB 2021 RIV US eng J - Článek v odborném periodiku
Dinesh, Dhurvas Chandrasekaran - Chalupská, Dominika - Šilhán, Jan - Koutná, Eliška - Nencka, Radim - Veverka, Václav - Bouřa, Evžen
Structural basis of RNA recognition by the SARS-CoV-2 nucleocapsid phosphoprotein.
PLoS Pathogens. Roč. 16, č. 12 (2020), č. článku e1009100. ISSN 1553-7366. E-ISSN 1553-7374
Grant CEP: GA MŠMT(CZ) EF16_019/0000729
Grant ostatní: AV ČR(CZ) L200551951
Institucionální podpora: RVO:61388963
Klíčová slova: N-terminal domain * binding domain * crystal structure
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 6.823, rok: 2020
Způsob publikování: Open access
https://doi.org/10.1371/journal.ppat.1009100

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease 2019 (COVID-19). SARS-CoV-2 is a single-stranded positivesense RNA virus. Like other coronaviruses, SARS-CoV-2 has an unusually large genome that encodes four structural proteins and sixteen nonstructural proteins. The structural nucleocapsid phosphoprotein N is essential for linking the viral genome to the viral membrane. Both N-terminal RNA binding (N-NTD) and C-terminal dimerization domains are involved in capturing the RNA genome and, the intrinsically disordered region between these domains anchors the ribonucleoprotein complex to the viral membrane. Here, we characterized the structure of the N-NTD and its interaction with RNA using NMR spectroscopy. We observed a positively charged canyon on the surface of the N-NTD that might serve as a putative RNA binding site similarly to other coronaviruses. The subsequent NMR titrations using single-stranded and double-stranded RNA revealed a much more extensive U-shaped RNA-binding cleft lined with regularly distributed arginines and lysines. The NMR data supported by mutational analysis allowed us to construct hybrid atomic models of the N-NTD/RNA complex that provided detailed insight into RNA recognition.
Trvalý link: http://hdl.handle.net/11104/0316257