Počet záznamů: 1
Serotonin and its metabolites reduce oxidative stress in murine RAW264.7 macrophages and prevent inflammation
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SYSNO ASEP 0524249 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Serotonin and its metabolites reduce oxidative stress in murine RAW264.7 macrophages and prevent inflammation Tvůrce(i) Vašíček, Ondřej (BFU-R) ORCID, RID
Lojek, Antonín (BFU-R) RID
Číž, Milan (BFU-R) RID, ORCIDCelkový počet autorů 3 Zdroj.dok. Journal of Physiology and Biochemistry. - : Springer - ISSN 1138-7548
Roč. 76, č. 1 (2020), s. 49-60Poč.str. 12 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. ES - Španělsko Klíč. slova nitric-oxide-synthase ; necrosis-factor-alpha ; human t-cells ; n-acetylserotonin Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP LD14030 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora BFU-R - RVO:68081707 UT WOS 000520009600004 EID SCOPUS 85077282839 DOI 10.1007/s13105-019-00714-3 Anotace In this study, we focused on comparing the effects of serotonin and its metabolites on the functions of RAW264.7 cells (emphasis on oxidative burst and production of nitric oxide and cytokines), thereby expanding the scope of existing knowledge with advent of novel findings in this field. Changes in production of reactive oxygen species (ROS) by RAW264.7 cells after treatment with serotonin, N-acetylserotonin and melatonin were determined using the chemiluminescence (CL) assay. To exclude the direct scavenging effects of the studied compounds on the CL response, the antioxidant properties of all respective compounds were measured using TRAP and amperometrical method. Nitric oxide (NO) production was measured by Griess reagent and inducible NO synthase (iNOS) expression by Western blot. Cytokine production was assessed using the Mouse Cytokine Panel A Array kit and ELISA. We showed that all tested compounds were able to reduce oxidative stress, as well as inhibit production of inflammatory cytokines by macrophages. Of the tested compounds, serotonin and N-acetylserotonin were markedly better antioxidants than melatonin. In comparison, other effects of tested compounds were very similar. It can be concluded that antioxidant capacity of tested compounds is a major advantage in the early stages of inflammation. Since plasma concentrations of N-acetylserotonin and melatonin are lower than serotonin, it can be deduced that serotonin plays a key role in modulation of inflammation and the regulatory functions of immune cells, while also protecting cells against oxidative stress. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2021 Elektronická adresa https://link.springer.com/article/10.1007/s13105-019-00714-3
Počet záznamů: 1