Počet záznamů: 1
Distinct Spatiotemporal Distribution of Bacterial Toxin-Produced Cellular cAMP Differentially Inhibits Opsonophagocytic Signaling
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SYSNO ASEP 0519008 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Distinct Spatiotemporal Distribution of Bacterial Toxin-Produced Cellular cAMP Differentially Inhibits Opsonophagocytic Signaling Tvůrce(i) Hasan, Shakir (MBU-M)
Rahman, Waheed Ur (MBU-M) ORCID
Šebo, Peter (MBU-M) RID, ORCID
Osička, Radim (MBU-M) RID, ORCIDČíslo článku 362 Zdroj.dok. Toxins. - : MDPI - ISSN 2072-6651
Roč. 11, č. 6 (2019)Poč.str. 18 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova 3',5 '-cyclic adenosine monophosphate (cAMP) ; adenylate cyclase toxin ; edema toxin Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA19-12695S GA ČR - Grantová agentura ČR GA18-20621S GA ČR - Grantová agentura ČR LM2015064 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura EATRIS-CZ II - 90064 - Univerzita Palackého v Olomouci Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000475328000059 EID SCOPUS 85068571823 DOI 10.3390/toxins11060362 Anotace Myeloid phagocytes have evolved to rapidly recognize invading pathogens and clear them through opsonophagocytic killing. The adenylate cyclase toxin (CyaA) of Bordetella pertussis and the edema toxin (ET) of Bacillus anthracis are both calmodulin-activated toxins with adenylyl cyclase activity that invade host cells and massively increase the cellular concentrations of a key second messenger molecule, 3',5'-cyclic adenosine monophosphate (cAMP). However, the two toxins differ in the kinetics and mode of cell entry and generate different cAMP concentration gradients within the cell. While CyaA rapidly penetrates cells directly across their plasma membrane, the cellular entry of ET depends on receptor-mediated endocytosis and translocation of the enzymatic subunit across the endosomal membrane. We show that CyaA-generated membrane-proximal cAMP gradient strongly inhibits the activation and phosphorylation of Syk, Vav, and Pyk2, thus inhibiting opsonophagocytosis. By contrast, at similar overall cellular cAMP levels, the ET-generated perinuclear cAMP gradient poorly inhibits the activation and phosphorylation of these signaling proteins. Hence, differences in spatiotemporal distribution of cAMP produced by the two adenylyl cyclase toxins differentially affect the opsonophagocytic signaling in myeloid phagocytes. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://www.mdpi.com/2072-6651/11/6/362
Počet záznamů: 1