Počet záznamů: 1
Modulation of endocrine nuclear receptor activities by polyaromatic compounds present in fractionated extracts of diesel exhaust particles
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SYSNO ASEP 0507732 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Modulation of endocrine nuclear receptor activities by polyaromatic compounds present in fractionated extracts of diesel exhaust particles Tvůrce(i) Pěnčíková, K. (CZ)
Cigánek, M. (CZ)
Neca, J. (CZ)
Illes, P. (CZ)
Dvořák, Z. (CZ)
Vondráček, Jan (BFU-R) RID, ORCID
Machala, M. (CZ)Celkový počet autorů 7 Zdroj.dok. Science of the Total Environment. - : Elsevier - ISSN 0048-9697
Roč. 677, AUG 10 2019 (2019), s. 626-636Poč.str. 11 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova aryl-hydrocarbon receptor ; polycyclic aromatic-hydrocarbons ; estrogen-receptor ; cell-line ; in-vitro Vědní obor RIV EH - Ekologie - společenstva Obor OECD Ecology CEP GA16-17085S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora BFU-R - RVO:68081707 UT WOS 000468191200059 DOI 10.1016/j.scitotenv.2019.04.390 Anotace Organic pollutants associated with diesel exhaust particles (DEP), such as polycyclic aromatic hydrocarbons (PAHs) and their derivatives, may negatively impact human health. However, a comprehensive overview of their effects on endocrine nuclear receptor activities is still missing. Here, we evaluated the effects of extracts and chromatographic fractions (fractionated according to increasing polarity) of two standard reference materials derived from distinct types of diesel engines (SRM 2975, SRM 1650b), on activation of androgen receptor (AR), estrogen receptor alpha (ER alpha), peroxisome proliferator-activated receptor gamma (PPAR gamma), glucocorticoid receptor (GR) and thyroid receptor alpha (TR alpha), using human cell-based reporter gene assays. Neither DEP standard modulated AR or GR activities. Crude extracts and fractions of SRM 1650b and SRM 2975 suppressed ER alpha-mediated activity in the ER-CALUX (TM) assay, however, this effect could be partly linked to their cytotoxicity in this cell line. We observed that only SRM 2975 extract and its fractions were partial PPAR gamma inducers, while SRM 1650b extract was not active towards this receptor. Importantly, we found that both extracts and polar fractions of SRM activated TR alpha and significantly potentiated the activity of endogenous TR alpha ligand, triiodothyronine. Based on a detailed chemical analysis of both extracts and their polar fractions, we identified several oxygenated PAH derivatives, that were present at relatively high levels in the analyzed DEP standards, including 3-nitrobenzanthrone (3-NBA), anthracene-9,10-dione, phenanthrene-9,10-dione. 9H-fluoren-9-one or benzo[a] anthracene-7,12-dione, to activate TR alpha activity. Nevertheless, these compounds provided only a minor contribution to the overall TR alpha activity identified in polar fractions. This suggests that yet unidentified polar polyaromatic compounds associated with DEP may, apart from their known impact on the aryl hydrocarbon receptor or steroid signaling, deregulate activities of additional nuclear receptors, in particular of TR alpha. This illustrates the need to better characterize endocrine disrupting activities of DEP. (C) 2019 Elsevier B.V. All rights reserved. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2020 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0048969719319357?via%3Dihub
Počet záznamů: 1