Počet záznamů: 1
Heat-treated carbon coatings on poly (L-lactide) foils for tissue engineering
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SYSNO ASEP 0505657 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Heat-treated carbon coatings on poly (L-lactide) foils for tissue engineering Tvůrce(i) Lišková, Jana (FGU-C) RID, ORCID
Kasálková-Slepičková, N. (CZ)
Slepička, P. (CZ)
Švorčík, V. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCIDZdroj.dok. Materials Science & Engineering C-Materials for Biological Applications. - : Elsevier - ISSN 0928-4931
Roč. 100, Jul (2019), s. 117-128Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova degradable polymers ; carbon films ; thermal treatment ; patterned surfaces ; guided cell growth ; cell-material interaction Vědní obor RIV JJ - Ostatní materiály Obor OECD Nano-processes (applications on nano-scale) CEP GBP108/12/G108 GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora FGU-C - RVO:67985823 UT WOS 000466059700012 EID SCOPUS 85062366384 DOI 10.1016/j.msec.2019.02.105 Anotace Carbon-based materials have emerged as promising candidates for a wide variety of biomedical applications, including tissue engineering. We have developed a simple but unique technique for patterning carbon-based substrates in order to control cell adhesion, growth and phenotypic maturation. Carbon films were deposited on PLLA foils from distances of 3 to 7 cm. Subsequent heat-treatment (60 degrees C, 1 h) created lamellar structures with dimensions decreasing from micro- to nanoscale with increasing deposition distance. All carbon films improved the spreading and proliferation of human osteoblast-like MG 63 cells, and promoted the alignment of these cells along the lamellar structures. Similar alignment was observed in human osteoblast-like Saos-2 cells and in human dermal fibroblasts. Type I collagen fibers produced by Saos-2 cells and fibroblasts were also oriented along the lamellar structures. These structures increased the activity of alkaline phosphatase in Saos-2 cells. Carbon coatings also supported adhesion and growth of vascular endothelial and smooth muscle cells, particularly flatter non-heated carbon films. On these films, the continuity of the endothelial cell layer was better than on heat-treated lamellar surfaces. Heat-treated carbon-coated PLLA is therefore more suitable for bone and skin tissue engineering, while carbon-coated PLLA without heating is more appropriate for vascular tissue engineering. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2020 Elektronická adresa https://doi.org/10.1016/j.msec.2019.02.105
Počet záznamů: 1