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Appropriate nonmycorrhizal controls in arbuscular mycorrhiza research: a microbiome perspective
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SYSNO ASEP 0497178 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Appropriate nonmycorrhizal controls in arbuscular mycorrhiza research: a microbiome perspective Tvůrce(i) Gryndler, Milan (MBU-M) RID, ORCID
Šmilauer, P. (CZ)
Püschel, David (MBU-M) RID, ORCID
Bukovská, Petra (MBU-M) RID, ORCID
Hršelová, Hana (MBU-M) RID, ORCID
Hujslová, Martina (MBU-M) RID, ORCID
Gryndlerová, Hana (MBU-M) ORCID
Beskid, Olena (MBU-M) RID
Konvalinková, Tereza (MBU-M) RID, ORCID
Jansa, Jan (MBU-M) RID, ORCIDZdroj.dok. Mycorrhiza. - : Springer - ISSN 0940-6360
Roč. 28, 5-6 (2018), s. 435-450Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Appropriate nonmycorrhizal control ; Microbiome ; Pot experiment Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GAP504/12/1665 GA ČR - Grantová agentura ČR LK11224 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000445927000004 EID SCOPUS 85048748954 DOI 10.1007/s00572-018-0844-x Anotace Establishment of nonmycorrhizal controls is a classic and recurrent theme in mycorrhizal research. For decades, authors reported mycorrhizal plant growth/nutrition as compared to various nonmycorrhizal controls. In such studies, uncertainties remain about which nonmycorrhizal controls are most appropriate and, in particular, what effects the control inoculations have on substrate and root microbiomes. Here, different types of control and mycorrhizal inoculations were compared with respect to plant growth and nutrition, as well as the structure of root and substrate microbiomes, assessed by next-generation sequencing. We compared uninoculated (absolute) control to inoculation with blank pot culture lacking arbuscular mycorrhizal fungi, filtrate of that blank inoculum, and filtrate of complex pot-produced mycorrhizal inoculum. Those treatments were compared to a standard mycorrhizal treatment, where the previously sterilized substrate was inoculated with complex pot-produced inoculum containing Rhizophagus irregularis SYM5. Besides this, monoxenically produced inoculum of the same fungus was applied either alone or in combination with blank inoculum. The results indicate that the presence of mycorrhizal fungus always resulted in stimulation of Andropogon gerardii plant biomass as well as in elevated phosphorus content of the plants. The microbial (bacterial and fungal) communities developing in the differently inoculated treatments, however, differed substantially from each other and no control could be obtained comparable with the treatment inoculated with complex mycorrhizal inoculum. Soil microorganisms with significant biological competences that could potentially contribute to the effects of the various inoculants on the plants were detected in roots and in plant cultivation substrate in some of the treatments. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2019
Počet záznamů: 1