Počet záznamů: 1
Transcriptional response to organic compounds from diverse gasoline and biogasoline fuel emissions in human lung cells
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SYSNO ASEP 0493306 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Transcriptional response to organic compounds from diverse gasoline and biogasoline fuel emissions in human lung cells Tvůrce(i) Líbalová, Helena (UEM-P) RID
Rössner ml., Pavel (UEM-P) RID, ORCID
Vrbová, Kristýna (UEM-P)
Brzicová, Táňa (UEM-P)
Sikorová, Jitka (UEM-P)
Vojtíšek-Lom, M. (CZ)
Beránek, V. (CZ)
Kléma, J. (CZ)
Cigánek, M. (CZ)
Neča, J. (CZ)
Machala, M. (CZ)
Topinka, Jan (UEM-P) RID, ORCIDZdroj.dok. Toxicology in Vitro. - : Elsevier - ISSN 0887-2333
Roč. 48, apr (2018), s. 329-341Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova gasoline exhaust particles ; alternative fuels ; organic extracts ; gene expression profiling ; DNA damage response Vědní obor RIV DN - Vliv životního prostředí na zdraví Obor OECD Biochemistry and molecular biology CEP GBP503/12/G147 GA ČR - Grantová agentura ČR LO1508 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015073 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEM-P - RVO:68378041 UT WOS 000428605400033 EID SCOPUS 85041918546 DOI 10.1016/j.tiv.2018.02.002 Anotace Modern vehicles equipped with Gasoline Direct Injection (GDI) engine have emerged as an important source of particulate emissions potentially harmful to human health. We collected and characterized gasoline exhaust particles (GEPs) produced by neat gasoline fuel (E0) and its blends with 15% ethanol (E15), 25% n-butanol (n-But25) and 25% isobutanol (i-But25). To study the toxic effects of organic compounds extracted from GEPs, we analyzed gene expression profiles in human lung BEAS-2B cells. Despite the lowest GEP mass, n-But25 extract contained the highest concentration of polycyclic aromatic hydrocarbons (PAHs), while i-But25 extract the lowest. Gene expression analysis identified activation of the DNA damage response and other subsequent events (cell cycle arrest, modulation of extracellular matrix, cell adhesion, inhibition of cholesterol biosynthesis) following 4 h exposure to all GEP extracts. The i-But25 extract induced the most distinctive gene expression pattern particularly after 24 h exposure. Whereas E0, E15 and n-But25 extract treatments resulted in persistent stress signaling including DNA damage response, MAPK signaling, oxidative stress, metabolism of PAHs or pro-inflammatory response, i-But25 induced changes related to the metabolism of the cellular nutrients required for cell recovery. Our results indicate that i-But25 extract possessed the weakest genotoxic potency possibly due to the low PAH content. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2019
Počet záznamů: 1