Počet záznamů: 1
Mapping of Plasma Membrane Proteins Interacting With Arabidopsis thaliana Flotillin 2
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SYSNO ASEP 0492287 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Mapping of Plasma Membrane Proteins Interacting With Arabidopsis thaliana Flotillin 2 Tvůrce(i) Junková, P. (CZ)
Daněk, Michal (UEB-Q) ORCID
Kocourková, Daniela (UEB-Q) ORCID
Brouzdová, Jitka (UEB-Q)
Kroumanová, Kristýna (UEB-Q) ORCID
Zelazny, E. (FR)
Janda, Martin (UEB-Q) RID, ORCID
Hynek, R. (CZ)
Martinec, Jan (UEB-Q) RID, ORCID
Valentová, O. (CZ)Celkový počet autorů 10 Číslo článku 991 Zdroj.dok. Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 9, JUL 12 (2018)Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova hypersensitive-induced reaction ; independent endocytic pathway ; syringae pv. tomato ; mass-spectrometry ; affinity-purification ; lipid rafts ; cell-death ; h+-atpase ; in-vivo ; signal-transduction ; Arabidopsis flotillin 2 ; protein-protein interactions ; immunopurification ; mass spectrometry ; splitubiquitin yeast system ; plant-pathogen interaction ; water transport ; intracellular trafficking Vědní obor RIV ED - Fyziologie Obor OECD Cell biology CEP GA14-09685S GA ČR - Grantová agentura ČR 7AMB17FR005 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000438356800001 EID SCOPUS 85050825092 DOI 10.3389/fpls.2018.00991 Anotace Arabidopsis flotillin 2 (At5g25260) belongs to the group of plant flotillins, which are not well characterized. In contrast, metazoan flotillins are well known as plasma membrane proteins associated with membrane microdomains that act as a signaling hub. The similarity of plant and metazoan flotillins, whose functions most likely consist of affecting other proteins via protein-protein interactions, determines the necessity of detecting their interacting partners in plants. Nevertheless, identifying the proteins that form complexes on the plasma membrane is a challenging task due to their low abundance and hydrophobic character. Here we present an approach for mapping Arabidopsis thaliana flotillin 2 plasma membrane interactors, based on the immunoaffinity purification of crosslinked and enriched plasma membrane proteins with mass spectrometry detection. Using this approach, 61 proteins were enriched in the AtFlot-GFP plasma membrane fraction, and 19 of them were proposed to be flotillin 2 interaction partners. Among our proposed partners of Flot2, proteins playing a role in the plant response to various biotic and abiotic stresses were detected. Additionally, the use of the split-ubiquitin yeast system helped us to confirm that plasma-membrane ATPase 1, early-responsive to dehydration stress protein 4, syntaxin-71, harpin-induced protein-like 3, hypersensitive-induced response protein 2 and two aquaporin isoforms interact with flotillin 2 directly. Based on the results of our study and the reported properties of Flot2 interactors, we propose that Flot2 complexes may be involved in plant-pathogen interactions, water transport and intracellular trafficking. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2019
Počet záznamů: 1