Počet záznamů: 1
Monitoring lipid production in yeast using SEM and Raman spectroscopy
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SYSNO ASEP 0481587 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název Monitoring lipid production in yeast using SEM and Raman spectroscopy Tvůrce(i) Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Šiler, Martin (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Hároniková, A. (CZ)
Marová, I. (CZ)Celkový počet autorů 7 Zdroj.dok. 13th Multinational Congress on Microscopy: Book of Abstracts. - Zagreb : Ruder Bošković Institute, Croatian Microscopy Society, 2017 / Gajović A. ; Weber I. ; Kovačević G. ; Čadež V. ; Šegota S. ; Peharec Štefanić P. ; Vidoš A. - ISBN 978-953-7941-19-2 Rozsah stran s. 308-309 Poč.str. 2 s. Forma vydání Tištěná - P Akce Multinational Congress on Microscopy /13./ Datum konání 24.09.2017 - 29.09.2017 Místo konání Rovinj Země HR - Chorvatsko Typ akce WRD Jazyk dok. eng - angličtina Země vyd. HR - Chorvatsko Klíč. slova yeast ; lipid bodies ; SEM ; Raman spectroscopy Vědní obor RIV BH - Optika, masery a lasery Obor OECD Electrical and electronic engineering CEP GA15-20645S GA ČR - Grantová agentura ČR LO1212 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED0017/01/01 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UPT-D - RVO:68081731 Anotace The main goal of our project is the development of specialized instrumentation and methodology to monitor metabolic states of microorganisms in order to optimize cultivation process for biotechnological applications. Here the attention is given to the oil-producing yeast strain Metschnikowia pulcherrima. The influence of different cultivation parameters (such as the effects of temperature regime and medium composition) on cells can be monitored. In our investigations on yeast cells scanning electron microscopy (SEM) and Raman spectroscopy techniques were used. Here, SEM uses electron beam to gain information about morphology of cells which reflects cells response on the different cultivation conditions. Consequently, Raman spectroscopy was used for the determination of lipids/oil present in the biomass. Thus, our study targets some factors which could lead to efficient industrial production of oil in selected biotechnological production. The yeast strains (Metschnikowia) were cultivated in medium containing different ratios of C/N (glycerol/yeast extract and/or ammonium sulphate) during a time period of two weeks. On the different days of cultivation, the cells were collected by centrifugation (2000 rpm, 1 min.) and analysed. SEM - the samples of yeast were cultivated as mentioned above and observed by cryo scanning electron microscopy (cryo-SEM). Pracoviště Ústav přístrojové techniky Kontakt Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Rok sběru 2018
Počet záznamů: 1