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Extracellular Matrix Hydrogel Derived from Human Umbilical Cord as a Scaffold for Neural Tissue Repair and Its Comparison with Extracellular Matrix from Porcine Tissues
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SYSNO ASEP 0478558 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Extracellular Matrix Hydrogel Derived from Human Umbilical Cord as a Scaffold for Neural Tissue Repair and Its Comparison with Extracellular Matrix from Porcine Tissues Tvůrce(i) Kočí, Zuzana (UEM-P)
Výborný, Karel (UEM-P)
Dubišová, Jana (UEM-P)
Vacková, Irena (UEM-P)
Jäger, Aleš (FZU-D) RID, ORCID
Lunov, Oleg (FZU-D) ORCID
Jiráková, Klára (UEM-P)
Kubinová, Šárka (UEM-P) RID, ORCIDZdroj.dok. Tissue Engineering: Part C-Methods - ISSN 1937-3384
Roč. 23, č. 6 (2017), s. 333-345Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova extracellular matrix ; hydrogel ; umbilical cord Vědní obor RIV FH - Neurologie, neurochirurgie, neurovědy Obor OECD Neurosciences (including psychophysiology Vědní obor RIV – spolupráce Fyzikální ústav - Genetika a molekulární biologie CEP GA15-01396S GA ČR - Grantová agentura ČR LO1309 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF15_003/0000419 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Fellowship J. E. Purkyně GA AV ČR - Akademie věd Institucionální podpora UEM-P - RVO:68378041 ; FZU-D - RVO:68378271 UT WOS 000403546300002 DOI 10.1089/ten.tec.2017.0089 Anotace Extracellular matrix (ECM) hydrogels prepared by tissue decellularization have been reported as natural injectable materials suitable for neural tissue repair. In this study, we prepared ECM hydrogel derived from human umbilical cord (UC) and evaluated its composition and mechanical and biological properties in comparison with the previously described ECM hydrogels derived from porcine urinary bladder (UB), brain, and spinal cord. The ECM hydrogels did not differ from each other in the concentration of collagen, while the highest content of glycosaminoglycans as well as the shortest gelation time was found for UC-ECM. The elastic modulus was then found to be the highest for UB-ECM. In spite of a different origin, topography, and composition, all ECM hydrogels similarly promoted the migration of human mesenchymal stem cells (MSCs) and differentiation of neural stem cells, as well as axonal outgrowth in vitro. However, only UC-ECM significantly improved proliferation of tissue-specific UC-derived MSCs when compared with the other ECMs. Injection of UC-ECM hydrogels into a photothrombotic cortical ischemic lesion in rats proved its in vivo gelation and infiltration with host macrophages. In summary, this study proposes UC-ECM hydrogel as an easily accessible biomaterial of human origin, which has the potential for neural as well as other soft tissue reconstruction. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2018
Počet záznamů: 1