Počet záznamů: 1
Separation of oligopeptides, nucleobases, nucleosides and nucleotides using capillary electrophoresis/electrochromatography with sol–gel modified inner capillary wall
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SYSNO ASEP 0478035 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Separation of oligopeptides, nucleobases, nucleosides and nucleotides using capillary electrophoresis/electrochromatography with sol–gel modified inner capillary wall Tvůrce(i) Svobodová, Jana (FGU-C)
Kofroňová, Olga (MBU-M) RID, ORCID
Benada, Oldřich (MBU-M) ORCID, RID
Král, V. (CZ)
Mikšík, Ivan (FGU-C) RID, ORCID, SAIZdroj.dok. Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1517, Sep 29 (2017), s. 185-194Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova capillary electrochromatography (CEC) ; open-tubular capillary electrochromatography (OT-CEC) ; nucleo-compounds ; oligopeptides ; sol–gel methods ; Porphyrin ; scanning electron microscopy (SEM) Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry Vědní obor RIV – spolupráce Mikrobiologický ústav - Biochemie CEP GA15-01948S GA ČR - Grantová agentura ČR LO1509 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora FGU-C - RVO:67985823 ; MBU-M - RVO:61388971 UT WOS 000411771500020 EID SCOPUS 85028336446 DOI 10.1016/j.chroma.2017.08.014 Anotace The aim of this article is to study the modification of an inner capillary wall with sol–gel coating (pure silica sol–gel or silica sol–gel containing porphyrin-brucine conjugate) and determine its influence on the separation process using capillary electrophoresis/electrochromatography method. After modification of the inner capillary surface the separation of analytes was performed using two different phosphate buffers (pH 2.5 and 9.0) and finally the changes in electrophoretic mobilities of various samples were calculated. To confirm that the modification of the inner capillary surface was successful, the parts of the inner surfaces of capillaries were observed using scanning electron microscopy. The analytes used as testing samples were oligopeptides, nucleosides, nucleobases and finally nucleotides. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2018
Počet záznamů: 1