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Physiology of spontaneous [Ca2+](i) oscillations in the isolated vasopressin and oxytocin neurones of the rat supraoptic nucleus
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SYSNO ASEP 0460348 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Physiology of spontaneous [Ca2+](i) oscillations in the isolated vasopressin and oxytocin neurones of the rat supraoptic nucleus Tvůrce(i) Kortus, Štěpán (FGU-C) ORCID
Srinivasan, Ch. (CZ)
Forostyak, O. (CZ)
Ueta, Y. (JP)
Syková, E. (CZ)
Chvátal, A. (CZ)
Zápotocký, Martin (FGU-C) RID, ORCID
Verkhratsky, A. (GB)
Dayanithi, G. (CZ)Zdroj.dok. Cell Calcium. - : Elsevier - ISSN 0143-4160
Roč. 59, č. 6 (2016), s. 280-288Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova magnocellular neurosecretory cells ; supraoptic nucleus ; vasopressin ; oxytocin ; transgenic rats ; Ca2+ oscillations Vědní obor RIV FH - Neurologie, neurochirurgie, neurovědy CEP GBP304/12/G069 GA ČR - Grantová agentura ČR Institucionální podpora FGU-C - RVO:67985823 UT WOS 000376831800002 EID SCOPUS 84963543381 DOI 10.1016/j.ceca.2016.04.001 Anotace The magnocellular vasopressin (AVP) and oxytocin (OT) neurones exhibit specific electrophysiological behaviour, synthesise AVP and OT peptides and secrete them into the neurohypophysial system in response to various physiological stimulations. The activity of these neurones is regulated by the very same peptides released either somato-dendritically or when applied to supraoptic nucleus (SON) preparations in vitro. The AVP and OT, secreted somato-dendritically (i.e. in the SON proper) act through specific autoreceptors, induce distinct Ca2+ signals and regulate cellular events. Here, we demonstrate that about 70% of freshly isolated individual SON neurones from the adult non-transgenic or transgenic rats bearing AVP (AVP-eGFP) or OT (OT-mRFP1) markers, produce distinct spontaneous [Ca2+](i) oscillations. In the neurones identified (through specific fluorescence), about 80% of AVP neurones and about 60% of OT neurones exhibited these oscillations. Exposure to AVP triggered [Ca2+](i) oscillations in silent AVP neurones, or modified the oscillatory pattern in spontaneously active cells. Hyper-and hypo-osmotic stimuli (325 or 275 mOsmol/l) respectively intensified or inhibited spontaneous [Ca2+](i) dynamics. In rats dehydrated for 3 or 5 days almost 90% of neurones displayed spontaneous [Ca2+](i) oscillations. More than 80% of OT-mRFP1 neurones from 3 to 6-day-lactating rats were oscillatory vs. about 44% (OT-mRFP1 neurones) in virgins. Together, these results unveil for the first time that both AVP and OT neurones maintain, via Ca2+ signals, their remarkable intrinsic in vivo physiological properties in an isolated condition.
Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2017
Počet záznamů: 1