Počet záznamů: 1
Comparative growth characterization of frequently used substrains of the model cyanobacterium Synechocystis sp. PCC 6803 under varying culture conditions
- 1.
SYSNO ASEP 0456348 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název Comparative growth characterization of frequently used substrains of the model cyanobacterium Synechocystis sp. PCC 6803 under varying culture conditions Tvůrce(i) Zavřel, Tomáš (UEK-B) RID, SAI, ORCID
Očenášová, Petra (UEK-B) SAI
Sinětova, M. A. (RU)
Červený, Jan (UEK-B) RID, ORCID, SAIZdroj.dok. Global Change: A Complex Challenge : Conference Proceedings. - Brno : Global Change Research Centre, The Czech Academy of Sciences, v. v. i., 2015 / Urban Otmar ; Šprtová Mirka ; Klem Karel - ISBN 978-80-87902-10-3 Rozsah stran s. 154-157 Poč.str. 157 s. Forma vydání Tištěná - P Akce Global Change: A Complex Challenge /4th/ Datum konání 23.03.2015-24.03.2015 Místo konání Brno Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova growth characterization ; cyanobacterium Synechocystis ; culture conditions Vědní obor RIV EH - Ekologie - společenstva CEP LO1415 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0073 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EE2.3.20.0256 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora RVO:67179843 - RVO:67179843 UT WOS 000381161600037 Anotace Cyanobacteria have gained increased attention as ideal candidates for biotechnological applications due to their capacity to produce valuable molecules ranging from therapeutic proteins to biofuels. Their natural phenotypic plasticity in highly dynamic environments enables easy deployment of new biotechnologies as well as opening possibilities for genetic engineering. This contribution presents a new approach to fast and reliable characterization of cyanobacteria growth in a flat panel photobioreactor that enables examination of changing light, temperature, and nutrient availability. The utilization of semi-continuous automatic cultivation with real-time culture growth monitoring provides a strong experimental basis for both characterization and optimization of cyanobacteria cultures in photobioreactors. We first characterized the autotrophic growth of the substrain of Synechocystis sp. PCC 6803 denoted as GT-L. This strain is capable of efficient growth under a wide range of environmental conditions with doubling time as fast as 5 h under favorable conditions. However, differences among Synechocystis sp. PCC 6803 substrains have been identified on both the genotype and phenotype levels. We therefore aimed to utilize the experimental platform to characterize multiple commonly used Synechocystis sp. PCC 6803 substrains. This method will enable us to identify substrains capable of robust growth and high production yields as reliable biotechnological candidates. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2016
Počet záznamů: 1