Novel high-affinity binders of human interferon gamma derived from albumin-binding domain of protein G

Ahmad, Jawid Nazir; Li, J.; Biedermannová, Lada; Kuchař, Milan; Šípová, Hana; Semerádtová, A.; Černý, Jiří; Petroková, Hana; Mikulecký, Pavel; Polínek, Jiří; Staněk, Ondřej; Vondrášek, Jiří; Homola, Jiří; Malý, J.; Osička, Radim; Šebo, Peter; Malý, Petr

doi:https://dx.doi.org/10.1002/prot.23234

Počet záznamů: 1

Novel high-affinity binders of human interferon gamma derived from albumin-binding domain of protein G

1.

SYSNO ASEP	0385253
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Novel high-affinity binders of human interferon gamma derived from albumin-binding domain of protein G
Tvůrce(i)	Ahmad, Jawid Nazir (MBU-M)_RID Li, J. (CN) Biedermannová, Lada (BTO-N)_{RID, ORCID} Kuchař, Milan (BTO-N)_RID Šípová, Hana (URE-Y) Semerádtová, A. (CZ) Černý, Jiří (BTO-N)_{RID, ORCID} Petroková, Hana (BTO-N)_RID Mikulecký, Pavel (BTO-N)_RID Polínek, Jiří (BTO-N) Staněk, Ondřej (MBU-M)_{RID, ORCID} Vondrášek, Jiří (BTO-N) Homola, Jiří (URE-Y)_RID Malý, J. (CZ) Osička, Radim (MBU-M)_{RID, ORCID} Šebo, Peter (MBU-M)_{RID, ORCID} Malý, Petr (BTO-N)_{RID, ORCID}
Zdroj.dok.	Proteins-Structure, Function and Bioinformatics. - : Wiley - ISSN 0887-3585 Roč. 80, č. 3 (2012), s. 774-789
Poč.str.	16 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	recombinant ligand ; protein scaffold ; computational design
Vědní obor RIV	CE - Biochemie
CEP	KAN200520702 GA AV ČR - Akademie věd
	GAP305/10/2184 GA ČR - Grantová agentura ČR
CEZ	AV0Z50200510 - MBU-M (2005-2011)
	AV0Z50520701 - BTO-N (2007-2013)
	AV0Z20670512 - URE-Y (2005-2011)
UT WOS	000300053500026
DOI	10.1002/prot.23234
Anotace	Recombinant ligands derived from small protein scaffolds show promise as robust research and diagnostic reagents and next generation protein therapeutics. Here, we derived high-affinity binders of human interferon gamma (hIFN gamma) from the three helix bundle scaffold of the albumin-binding domain (ABD) of protein G from Streptococcus G148. Computational interaction energy mapping, solvent accessibility assessment, and in silico alanine scanning identified 11 residues from the albumin-binding surface of ABD as suitable for randomization. A corresponding combinatorial ABD scaffold library was synthesized and screened for hIFN gamma binders using in vitro ribosome display selection, to yield recombinant ligands that exhibited Kd values for hIFN gamma from 0.2 to 10 nM
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2013

Počet záznamů: 1