Počet záznamů: 1
Molecular differentiation of three loach species (Pisces, Cobitidae) based on the nuclear 5S rDNA marker
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SYSNO ASEP 0384520 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Molecular differentiation of three loach species (Pisces, Cobitidae) based on the nuclear 5S rDNA marker Tvůrce(i) Kirtiklis, L. (PL)
Boron, A. (PL)
Ptasznik, P. (PL)
Lusková, Věra (UBO-W) RID, SAI
Lusk, Stanislav (UBO-W) RIDCelkový počet autorů 5 Zdroj.dok. Folia Biologica-Krakow - ISSN 0015-5497
Roč. 59, 3-4 (2011), s. 141-145Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. PL - Polsko Klíč. slova Cobitis ; PCR-RFLP ; Sabanejewia ; species identification ; 5S rDNA Vědní obor RIV EB - Genetika a molekulární biologie CEZ AV0Z60930519 - UBO-W (2005-2011) UT WOS 000293365100009 EID SCOPUS 80051706363 DOI 10.3409/fb59_3-4.141-145 Anotace The diversity of the 5S rDNA fragment among three loach species: Cobitis taenia, C. elongatoides and Sabanejewia aurata was investigated using universal PCR primers for this gene. Three amplification products were obtained: 220 bp length for C. taenia and C. elongatoides, and 330 bp for S. aurata. Two amplicons with the same length (in Cobitis) were digested with TaqI restriction endonuclease. This enzyme found one restriction site T/CGA in the C. elongatoides fragment, while in the case of C. taenia no cleavage effect was observed. On this basis we constructed an easy and cheap method for loach species discrimination. It seems adequate for effective support of conservation initiatives for endangered loathes. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2013
Počet záznamů: 1