Počet záznamů: 1
Do Phytotropins Inhibit Auxin Efflux by Impairing Vesicle Traffic?
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SYSNO ASEP 0172372 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Do Phytotropins Inhibit Auxin Efflux by Impairing Vesicle Traffic? Tvůrce(i) Petrášek, Jan (UEB-Q) RID, ORCID
Černá, A. (CZ)
Schwarzerová, K. (CZ)
Elčkner, Miroslav (UEB-Q)
Morris, David (UEB-Q)
Zažímalová, Eva (UEB-Q) RID, ORCIDZdroj.dok. Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 131, č. 1 (2003), s. 254-263Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova 1-N-naphthylphthalamic acid ; BY-2 tobacco ; auxin efflux Vědní obor RIV EB - Genetika a molekulární biologie CEP LN00A081 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z5038910 - UEB-Q Anotace Phytotropins such as 1-N-naphthylphthalamic acid (NPA) strongly inhibit auxin efflux, but the mechanism of this inhibition remains unknown. Auxin efflux is also strongly decreased by the vesicle trafficking inhibitor brefeldin A (BFA). Using suspension-cultured interphase cells of the BY-2 tobacco (Nicotiana tabacum L. cv Bright-Yellow 2) cell line, we compared the effects of NPA and BFA on auxin accumulation and on the arrangement of the cytoskeleton and endoplasmic reticulum (ER). The inhibition of auxin efflux (stimulation of net accumulation) by both NPA and BFA occurred rapidly with no measurable lag. NPA had no observable effect on the arrangement of microtubules, actin filaments, or ER. Thus, its inhibitory effect on auxin efflux was not mediated by perturbation of the cytoskeletal system and ER. BFA, however, caused substantial alterations to the arrangement of actin filaments and ER, including a characteristic accumulation of actin in the perinuclear cytoplasm. Even at saturating concentrations, NPA inhibited net auxin efflux far more effectively than did BFA. Therefore, a proportion of the NPA-sensitive auxin efflux carriers may be protected from the action of BFA. Maximum inhibition of auxin efflux occurred at concentrations of NPA substantially below those previously reported to be necessary to perturb vesicle trafficking. We found no evidence to support recent suggestions that the action of auxin transport inhibitors is mediated by a general inhibition of vesicle-mediated protein traffic to the plasma membrane. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2004
Počet záznamů: 1