Počet záznamů: 1  

Terbinafine resistance in Trichophyton mentagrophytes and Trichophyton rubrum in the Czech Republic: A prospective multicentric study

  1. 1.
    0584095 - MBÚ 2025 RIV US eng J - Článek v odborném periodiku
    Kolarczyková, D. - Lysková, P. - Švarcová, Michaela - Kuklová, I. - Dobiáš, R. - Mallátová, N. - Kolařík, Miroslav - Hubka, Vít
    Terbinafine resistance in Trichophyton mentagrophytes and Trichophyton rubrum in the Czech Republic: A prospective multicentric study.
    Mycoses. Roč. 67, č. 2 (2024), č. článku e13708. ISSN 0933-7407. E-ISSN 1439-0507
    Grant CEP: GA MZd(CZ) NU21-05-00681
    Grant ostatní: AV ČR(CZ) StrategieAV21/33
    Program: StrategieAV
    Institucionální podpora: RVO:61388971
    Klíčová slova: dermatophytosis * allylamines * anthropophilic dermatophytes * antifungal resistance * dermatomycosis * squalene epoxidase * zoophilic dermatophytes
    Obor OECD: Microbiology
    Impakt faktor: 4.9, rok: 2022
    Způsob publikování: Open access
    https://onlinelibrary.wiley.com/doi/10.1111/myc.13708

    Background: Terbinafine, an allylamine antifungal, is crucial for treating dermatophytosis by inhibiting squalene epoxidase (SQLE) in the ergosterol biosynthetic pathway. However, resistance is emerging, particularly in India and Southeast Asia, but reports of resistance spread worldwide. Despite this, comprehensive studies on terbinafine resistance in Trichophyton are still limited. Objectives: This research aimed to determine the prevalence of terbinafine resistance in the Czech Republic, with a focus on Trichophyton rubrum and Trichophyton mentagrophytes, and investigate the underlying molecular mechanisms. Patients/Methods: A total of 514 clinical strains of T. rubrum and 240 T. mentagrophytes collected from four Czech clinical institutions were screened for terbinafine resistance. Molecular investigations included DNA sequencing, specifically the ITS rDNA region and SQLE gene, as well as antifungal susceptibility testing following EUCAST guidelines. Results: While no resistance was observed in T. rubrum, 2.5% of T. mentagrophytes strains exhibited resistance, marked by the F397L mutation in SQLE. Notably, resistance surged from 1.2% in 2019 to 9.3% in 2020 but reverted to 0% in 2021. All resistant strains were identified as T. mentagrophytes var. indotineae. Resistant strains exhibited high MICs for terbinafine (>= 4 mg L-1) but low MICs to the other seven antifungals tested except for fluconazole. Conclusions: This study highlights the emergence of terbinafine-resistant T. mentagrophytes strains in the Czech Republic, with the F397L mutation being pivotal. Due to the relatively low resistance level, the current guidelines for dermatomycosis treatment in the Czech Republic remain effective, but ongoing surveillance is essential for timely adaptations if resistance patterns change.
    Trvalý link: https://hdl.handle.net/11104/0352119

     
     
Počet záznamů: 1  

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