Počet záznamů: 1
Evaluation of solid-phase extraction sorbents for purification of oligosaccharides and glycans derivatized by positively charged labels followed by capillary electrophoretic analysis
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SYSNO ASEP 0580317 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Evaluation of solid-phase extraction sorbents for purification of oligosaccharides and glycans derivatized by positively charged labels followed by capillary electrophoretic analysis Tvůrce(i) Smolková, Denisa (UIACH-O)
Moravcová, Dana (UIACH-O) RID, ORCID
Lavická, Jana (UIACH-O) ORCID, RIDCelkový počet autorů 3 Zdroj.dok. Journal of Separation Science. - : Wiley - ISSN 1615-9306
(2024), s. 1-11Poč.str. 11 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova sample preparation ; protein glycosylation ; n-glycans ; fluorescence ; capillary electrophoresis ; solid-phase extraction Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GA22-00236S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UIACH-O - RVO:68081715 UT WOS 001125781200001 EID SCOPUS 85179715048 DOI 10.1002/jssc.202300705 Anotace The sample preparation including labeling and clean-up represents a key analytical step in the analysis of oligosaccharides and glycans by either chromatographic or electrophoretic separation methods. Although the majority of labeling has been performed by neutral and/or negatively charged tags, the introduction of a positive charge into the saccharide molecule can significantly improve the analysis, especially with mass spectrometry detection. In this work, we present the evaluation of five solid-phase extraction sorbents differing in extraction chemistry for the clean-up and concentration of positively labeled maltooligosaccharides from the reaction mixtures. Maltooligosaccharides containing four to seven glucose units were labeled by cationic tags (2-aminoethyl)trimethylammonium chloride and (carboxymethyl)trimethylammonium chloride hydrazide and the extraction conditions were optimized followed by electrophoretic analysis with conductivity detection. The effects of the solid-phase extraction sorbent chemistry, extraction conditions, and sample composition are discussed. All tested sorbents were capable of cleaning up maltooligosaccharides from the reaction mixtures to some extent after optimization of the solid-phase extraction procedure (51.9%-98.9% recovery). The best-rated amide-based sorbent was used to process the sample of N-linked glycans enzymatically released from ribonuclease B. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2025 Elektronická adresa https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jssc.202300705
Počet záznamů: 1