Počet záznamů: 1
AAK1-like: A putative pseudokinase with potential roles in cargo uptake in bloodstream form iTrypanosoma brucei/i parasites
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SYSNO ASEP 0579861 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název AAK1-like: A putative pseudokinase with potential roles in cargo uptake in bloodstream form iTrypanosoma brucei/i parasites Tvůrce(i) Black, J. (GB)
Klinger, C.M. (GB)
Lemgruber, L. (GB)
Dacks, Joel Bryan (BC-A) SAI, ORCID
Mottram, J.C. (GB)
McCulloch, R. (GB)Celkový počet autorů 6 Číslo článku e12994 Zdroj.dok. Journal of Eukaryotic Microbiology. - : Wiley - ISSN 1066-5234
Roč. 70, DEC (2023)Poč.str. 20 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova clathrin-mediated endocytosis ; flagellar pocket ; structural explanation ; phylogenetic analysis ; protein ; surface ; ap-2 ; identification ; recognition ; subunit ; aak1 ; African trypanosomes ; AP-2 complex ; endocytosis Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 001043849700001 EID SCOPUS 85167344778 DOI 10.1111/jeu.12994 Anotace Selection and internalization of cargo via clathrin-mediated endocytosis requires adaptor protein complexes. One complex, AP-2, acts during cargo selection at the plasma membrane. African trypanosomes lack all components of the AP-2 complex, except for a recently identified orthologue of the AP-2-associated protein kinase 1, AAK1. In characterized eukaryotes, AAK1 phosphorylates the mu 2 subunit of the AP-2 complex to enhance cargo recognition and uptake into clathrin-coated vesicles. Here, we show that kinetoplastids encode not one, but two AAK1 orthologues: one (AAK1L2) is absent from salivarian trypanosomes, while the other (AAK1L1) lacks important kinase-specific residues in a range of trypanosomes. These AAK1L1 and AAK1L2 novelties reinforce suggestions of functional divergence in endocytic uptake within salivarian trypanosomes. Despite this, we show that AAK1L1 null mutant Trypanosoma brucei, while viable, display slowed proliferation, morphological abnormalities including swelling of the flagellar pocket, and altered cargo uptake. In summary, our data suggest an unconventional role for a putative pseudokinase during endocytosis and/or vesicular trafficking in T. brucei, independent of AP-2. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2024 Elektronická adresa https://onlinelibrary.wiley.com/doi/10.1111/jeu.12994
Počet záznamů: 1