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Artificial Intelligence-Aided Massively Parallel Spectroscopy of Freely Diffusing Nanoscale Entities
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SYSNO ASEP 0574423 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Artificial Intelligence-Aided Massively Parallel Spectroscopy of Freely Diffusing Nanoscale Entities Tvůrce(i) Hlaváček, Antonín (UIACH-O) ORCID
Uhrová, Kateřina (UIACH-O)
Weisová, Julie (UIACH-O)
Křivánková, Jana (UIACH-O) RID, ORCIDCelkový počet autorů 4 Zdroj.dok. Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 95, č. 33 (2023), s. 12256-12263Poč.str. 8 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova photon-upconversion ; fluorescence cross-correlation spectroscopy ; slitless spectroscopy ; massively parallel spectroscopy Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry Způsob publikování Open access Institucionální podpora UIACH-O - RVO:68081715 UT WOS 001043684300001 EID SCOPUS 85168364256 DOI 10.1021/acs.analchem.3c01043 Anotace Massively parallel spectroscopy (MPS) of many single nanoparticles in an aqueous dispersion is reported. As a model system, bioconjugated photon-upconversion nanoparticles (UCNPs) with a near-infrared excitation are prepared. The UCNPs are doped either with Tm3+ (emission 450 and 802 nm) or Er3+ (emission 554 and 660 nm). Particles emitting two emission wavelengths appear as double spots in the MPS images. The counting of double spots per MPS image is principally comparable to the cross-correlation amplitude in cross-correlation spectroscopy─both quantities can be used for quantification. However, MPS possesses much larger detection volumes and operates digitally, MPS can scan approximately 103× larger volume of dispersion in the same unit of time. These UCNPs are conjugated to biotinylated bovine serum albumin (Tm3+-doped) or streptavidin (Er3+-doped). MPS is correlated with an ensemble spectra measurement, and the limit of detection (1.6 fmol L–1) and the linearity range (4.8 fmol L–1 to 40 pmol L–1) for bioconjugated UCNPs are estimated. MPS is used for observing the bioaffinity clustering of bioconjugated UCNPs. This observation is correlated with a native electrophoresis and bioaffinity assay on a microtiter plate. A competitive MPS bioaffinity assay for biotin is developed and characterized with a limit of detection of 6.6 nmol L–1. MPS from complex biological matrices (cell cultivation medium) is performed without increasing background. The compatibility with polydimethylsiloxane microfluidics is proven by recording MPS from a 30 μm deep microfluidic channel. Because MPS is a new technique, the applications are only limited by the imagination and the persistence of the experimenter. Besides characterizing freely diffusing molecules and nanoparticles of diverse types, we speculate on homogeneous immunochemical assays and ratiometric nanosensors for high-throughput microfluidics. Additional imaging modalities like fluorescence, dark-field, and bright-field are of high interest. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2024 Elektronická adresa https://pubs.acs.org/doi/epdf/10.1021/acs.analchem.3c01043
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