Počet záznamů: 1
Luminal Surface Plasma Treatment of Closed Cylindrical Microchannels: A Tool toward the Creation of On-Chip Vascular Endothelium
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SYSNO ASEP 0572386 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Luminal Surface Plasma Treatment of Closed Cylindrical Microchannels: A Tool toward the Creation of On-Chip Vascular Endothelium Tvůrce(i) Černík, Marek (BFU-R) ORCID
Poláková, Kamila (BFU-R)
Kubala, Lukáš (BFU-R) RID, ORCID
Wünschová, Andrea Vítečková (BFU-R)
Danylevska, A. M. G. (CZ)
Pešková, Michaela (BFU-R)
Víteček, Jan (BFU-R) RID, ORCIDCelkový počet autorů 7 Zdroj.dok. ACS BIOMATERIALS SCIENCE & ENGINEERING. - : American Chemical Society - ISSN 2373-9878
Roč. 9, č. 5 (2023), s. 2755-2763Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova 3D printing ; endothelial cell ; in vitro model ; plasma oxidation ; pdms ; surface modification Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials CEP GA21-01057S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000982471500001 EID SCOPUS 85156090211 DOI 10.1021/acsbiomaterials.2c00887 Anotace On-chip vascular microfluidic models provide a great tool to study aspects of cardiovascular diseases in vitro. To produce such models, polydimethylsiloxane (PDMS) has been the most widely used material. For biological applications, its hydrophobic surface has to be modified. The major approach has been plasma-based surface oxidation, which has been very challenging in the case of channels enclosed within a microfluidic chip. The preparation of the chip combined a 3D-printed mold with soft lithography and commonly available materials. We have introduced the high-frequency low-pressure air-plasma surface modification of seamless channels enclosed within a PDMS microfluidic chip. The plasma treatment modified the luminal surface more uniformly than in previous works. Such a setup enabled a higher degree of design freedom and a possibility of rapid prototyping. Further, plasma treatment in combination with collagen IV coating created a biomimetic surface for efficient adhesion of vascular endothelial cells as well as promoted long-term cell culture stability under flow. The cells within the channels were highly viable and showed physiological behavior, confirming the benefit of the presented surface modification. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2024 Elektronická adresa https://pubs.acs.org/doi/10.1021/acsbiomaterials.2c00887
Počet záznamů: 1