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Antiproliferative Activity of Buddleja saligna (Willd.) against Melanoma and In Vivo Modulation of Angiogenesis
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SYSNO ASEP 0566373 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Antiproliferative Activity of Buddleja saligna (Willd.) against Melanoma and In Vivo Modulation of Angiogenesis Tvůrce(i) Twilley, D. (ZA)
Thipe, V. C. (US)
Kishore, N. (IN)
Bloebaum, P. (US)
Roma-Rodrigues, C. (PT)
Baptista, J. P. V. (PT)
Fernandes, A. R. (PT)
Selepe, M. A. (ZA)
Langhansová, Lenka (UEB-Q) RID, ORCID
Katti, K. (ZA)
Lall, N. (ZA)Celkový počet autorů 11 Číslo článku 1497 Zdroj.dok. Pharmaceuticals. - : MDPI
Roč. 15, č. 12 (2022)Poč.str. 24 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova angiogenesis ; antiproliferative activity ; Buddleja saligna ; ex ovo YSM ; melanoma Obor OECD Medicinal chemistry Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000904178300001 EID SCOPUS 85144700552 DOI 10.3390/ph15121497 Anotace Melanoma cells secrete pro-angiogenic factors, which stimulates growth, proliferation and metastasis, and therefore are key therapeutic targets. Buddleja saligna (BS), and an isolated triterpenoid mixture (DT-BS-01) showed a fifty percent inhibitory concentration (IC50) of 33.80 ± 1.02 and 5.45 ± 0.19 µg/mL, respectively, against melanoma cells (UCT-MEL-1) with selectivity index (SI) values of 1.64 and 5.06 compared to keratinocytes (HaCat). Cyclooxygenase-2 (COX-2) inhibition was observed with IC50 values of 35.06 ± 2.96 (BS) and 26.40 ± 4.19 µg/mL (DT-BS-01). BS (30 µg/mL) significantly inhibited interleukin (IL)-6 (83.26 ± 17.60%) and IL-8 (100 ± 0.2%) production, whereas DT-BS-01 (5 µg/mL) showed 51.07 ± 2.83 (IL-6) and 0 ± 6.7% (IL-8) inhibition. Significant vascular endothelial growth factor (VEGF) inhibition, by 15.84 ± 4.54 and 12.21 ± 3.48%, respectively, was observed. In the ex ovo chick embryo yolk sac membrane assay (YSM), BS (15 µg/egg) significantly reduced new blood vessel formation, with 53.34 ± 11.64% newly formed vessels. Silver and palladium BS nanoparticles displayed noteworthy SI values. This is the first report on the significant anti-angiogenic activity of BS and DT-BS-01 and should be considered for preclinical trials as there are currently no US Food and Drug Administration (FDA) approved drugs to inhibit angiogenesis in melanoma. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2023 Elektronická adresa https://doi.org/10.3390/ph15121497
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