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LACTB induces cancer cell death through the activation of the intrinsic caspase-independent pathway in breast cancer
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SYSNO ASEP 0563865 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název LACTB induces cancer cell death through the activation of the intrinsic caspase-independent pathway in breast cancer Tvůrce(i) Gonzalez-Morena, Juan M. (UOCHB-X) ORCID
Escudeiro-Lopes, Sara (UOCHB-X)
Ferreira Mendes, Jessica Marianne (UOCHB-X)
Jakoubě, Pavel (UOCHB-X) ORCID, RID
Cutano, Valentina (UOCHB-X) ORCID
Vinaixa Forner, Judith (UOCHB-X)
Králová Viziová, Petra (UMG-J)
Hartmanová, Andrea (UMG-J)
Sedláček, Radislav (UMG-J) RID
Machado, Susana (UOCHB-X) ORCID
Malčeková, Beata (UOCHB-X)
Kečkéšová, Zuzana (UOCHB-X) ORCIDZdroj.dok. Apoptosis. - : Springer - ISSN 1360-8185
Roč. 28, 1/2 (2023), s. 186-198Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova LACTB ; apoptosis ; cell death ; caspases ; mitochondria ; breast cancer ; cell cycle arrest Obor OECD Biochemistry and molecular biology CEP GJ18-24473Y GA ČR - Grantová agentura ČR EF19_074/0016322 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LX22NPO5102 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018126 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF18_046/0015861 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UOCHB-X - RVO:61388963 ; UMG-J - RVO:68378050 UT WOS 000871864300001 EID SCOPUS 85141062801 DOI 10.1007/s10495-022-01775-4 Anotace Background LACTB was recently identified as a mitochondrial tumour suppressor that negatively affects cancer cell proliferation by inducing cell death and/or differentiation, depending on the cell type and tissue. However, the detailed mechanism underlying the LACTB-induced cancer cell death is largely unknown. Methods We used cell-based, either in 2D or 3D conditions, and in vivo experiments to understand the LACTB mechanisms. In this regard, protein array followed by an enrichment analysis, cell proliferation assays using different compounds, western blot analysis, flow cytometry and immunofluorescence were performed. Differences between quantitative variables following normal distribution were valuated using Student t test for paired or no-paired samples according to the experiment. For in vivo experiments differences in tumour growth were analyzed by 2-way ANOVA. Results We show, that LACTB expression leads to cell cycle arrest in G1 phase and increase of DNA oxidation that leads to activation of intrinsic caspase-independent cell death pathway. This is achieved by an increase of mitochondrial reactive oxygen species since early time points of LACTB induction. Conclusion Our work provides a deeper mechanistic insight into LACTB-mediated cancer-cell death and shows the dynamics of the cellular responses a particular tumor suppressive stimulus might evoke under different genetic landscapes. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2024 Elektronická adresa https://doi.org/10.1007/s10495-022-01775-4
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