Počet záznamů: 1
Anisaxins, helical antimicrobial peptides from marine parasites, kill resistant bacteria by lipid extraction and membrane disruption
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SYSNO ASEP 0559179 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Anisaxins, helical antimicrobial peptides from marine parasites, kill resistant bacteria by lipid extraction and membrane disruption Tvůrce(i) Roncevic, T. (HR)
Gerdol, M. (IT)
Mardirossian, M. (IT)
Males, M. (HR)
Cvjetan, S. (HR)
Benincasa, M. (IT)
Maravi, A. (HR)
Gajski, G. (HR)
Krce, L. (HR)
Aviani, I. (HR)
Hrabar, J. (HR)
Trumbic, Z. (HR)
Derks, M. (NL)
Pallavicini, A. (IT)
Weingarth, M. (NL)
Zorani, L. (HR)
Tossi, A. (IT)
Mladineo, Ivona (BC-A) RID, ORCIDCelkový počet autorů 18 Zdroj.dok. Acta Biomaterialia. - : Elsevier - ISSN 1742-7061
Roč. 146, JUL (2022), s. 131-144Poč.str. 14 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova helical antimicrobial peptides ; Anisakis ; Lipid extraction ; Molecular leakage ; Multi-drug resistant bacteria ; Parasites Vědní obor RIV JJ - Ostatní materiály Obor OECD Textiles Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000813340300002 EID SCOPUS 85129983092 DOI 10.1016/j.actbio.2022.04.025 Anotace An infecting and propagating parasite relies on its innate defense system to evade the host's immune response and to survive challenges from commensal bacteria. More so for the nematode Anisakis, a marine parasite that during its life cycle encounters both vertebrate and invertebrate hosts and their highly diverse microbiotas. Although much is still unknown about how the nematode mitigates the effects of these microbiota, its antimicrobial peptides likely play an important role in its survival. We identified anisaxins, the first cecropin-like helical antimicrobial peptides originating from a marine parasite, by mining available genomic and transcriptomic data for Anisakis spp. These peptides are potent bactericidal agents in vitro , selectively active against Gram-negative bacteria, including multi-drug resistant strains, at sub-micromolar concentrations. Their interaction with bacterial membranes was confirmed by solid state NMR (ssNMR) and is highly dependent on the peptide concentration as well as peptide to lipid ratio, as evidenced by molecular dynamics (MD) simulations. MD results indicated that an initial step in the membranolytic mode of action involves membrane bulging and lipid extraction, a novel mechanism which may underline the peptides' potency. Subsequent steps include membrane permeabilization leading to leakage of molecules and eventually cell death, but without visible macroscopic damage, as shown by atomic force microscopy and flow cytometry. This membranolytic antibacterial activity does not translate to cytotoxicity towards human peripheral blood mononuclear cells (HPBMCs), which was minimal at well above bactericidal concentrations, making anisaxins promising candidates for further drug development. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2023 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S1742706122002343?via%3Dihub
Počet záznamů: 1