Počet záznamů: 1  

Anisaxins, helical antimicrobial peptides from marine parasites, kill resistant bacteria by lipid extraction and membrane disruption

  1. 1.
    SYSNO ASEP0559179
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevAnisaxins, helical antimicrobial peptides from marine parasites, kill resistant bacteria by lipid extraction and membrane disruption
    Tvůrce(i) Roncevic, T. (HR)
    Gerdol, M. (IT)
    Mardirossian, M. (IT)
    Males, M. (HR)
    Cvjetan, S. (HR)
    Benincasa, M. (IT)
    Maravi, A. (HR)
    Gajski, G. (HR)
    Krce, L. (HR)
    Aviani, I. (HR)
    Hrabar, J. (HR)
    Trumbic, Z. (HR)
    Derks, M. (NL)
    Pallavicini, A. (IT)
    Weingarth, M. (NL)
    Zorani, L. (HR)
    Tossi, A. (IT)
    Mladineo, Ivona (BC-A) RID, ORCID
    Celkový počet autorů18
    Zdroj.dok.Acta Biomaterialia. - : Elsevier - ISSN 1742-7061
    Roč. 146, JUL (2022), s. 131-144
    Poč.str.14 s.
    Forma vydáníTištěná - P
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovahelical antimicrobial peptides ; Anisakis ; Lipid extraction ; Molecular leakage ; Multi-drug resistant bacteria ; Parasites
    Vědní obor RIVJJ - Ostatní materiály
    Obor OECDTextiles
    Způsob publikováníOpen access
    Institucionální podporaBC-A - RVO:60077344
    UT WOS000813340300002
    EID SCOPUS85129983092
    DOI10.1016/j.actbio.2022.04.025
    AnotaceAn infecting and propagating parasite relies on its innate defense system to evade the host's immune response and to survive challenges from commensal bacteria. More so for the nematode Anisakis, a marine parasite that during its life cycle encounters both vertebrate and invertebrate hosts and their highly diverse microbiotas. Although much is still unknown about how the nematode mitigates the effects of these microbiota, its antimicrobial peptides likely play an important role in its survival. We identified anisaxins, the first cecropin-like helical antimicrobial peptides originating from a marine parasite, by mining available genomic and transcriptomic data for Anisakis spp. These peptides are potent bactericidal agents in vitro , selectively active against Gram-negative bacteria, including multi-drug resistant strains, at sub-micromolar concentrations. Their interaction with bacterial membranes was confirmed by solid state NMR (ssNMR) and is highly dependent on the peptide concentration as well as peptide to lipid ratio, as evidenced by molecular dynamics (MD) simulations. MD results indicated that an initial step in the membranolytic mode of action involves membrane bulging and lipid extraction, a novel mechanism which may underline the peptides' potency. Subsequent steps include membrane permeabilization leading to leakage of molecules and eventually cell death, but without visible macroscopic damage, as shown by atomic force microscopy and flow cytometry. This membranolytic antibacterial activity does not translate to cytotoxicity towards human peripheral blood mononuclear cells (HPBMCs), which was minimal at well above bactericidal concentrations, making anisaxins promising candidates for further drug development.
    PracovištěBiologické centrum (od r. 2006)
    KontaktDana Hypšová, eje@eje.cz, Tel.: 387 775 214
    Rok sběru2023
    Elektronická adresahttps://www.sciencedirect.com/science/article/pii/S1742706122002343?via%3Dihub
Počet záznamů: 1  

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