Počet záznamů: 1
Revealing structural peculiarities of homopurine GA repetition stuck by i-motif clip
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SYSNO ASEP 0555432 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Revealing structural peculiarities of homopurine GA repetition stuck by i-motif clip Tvůrce(i) Novotný, A. (CZ)
Novotný, J. (CZ)
Kejnovská, Iva (BFU-R) RID, ORCID
Vorlíčková, Michaela (BFU-R) RID, ORCID
Fiala, R. (CZ)
Marek, R. (CZ)Celkový počet autorů 6 Zdroj.dok. Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048
Roč. 49, č. 20 (2021), s. 11425-11437Poč.str. 13 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova parallel-stranded dna ; molecular-dynamics ; circular-dichroism ; rna duplexes Obor OECD Biochemistry and molecular biology CEP EF15_003/0000477 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA19-17063S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000728384400008 EID SCOPUS 85121219609 DOI 10.1093/nar/gkab915 Anotace Non-canonical forms of nucleic acids represent challenging objects for both structure-determination and investigation of their potential role in living systems. In this work, we uncover a structure adopted by GA repetition locked in a parallel homoduplex by an i-motif. A series of DNA oligonucleotides comprising GAGA segment and C-3 clip is analyzed by NMR and CD spectroscopies to understand the sequence-structure-stability relationships. We demonstrate how the relative position of the homopurine GAGA segment and the C-3 clip as well as single-base mutations (guanine deamination and cytosine methylation) affect base pairing arrangement of purines, i-motif topology and overall stability. We focus on oligonucleotides C(3)GAGA and methylated GAGAC(3) exhibiting the highest stability and structural uniformity which allowed determination of high-resolution structures further analyzed by unbiased molecular dynamics simulation. We describe sequence-specific supramolecular interactions on the junction between homoduplex and i-motif blocks that contribute to the overall stability of the structures. The results show that the distinct structural motifs can not only coexist in the tight neighborhood within the same molecule but even mutually support their formation. Our findings are expected to have general validity and could serve as guides in future structure and stability investigations of nucleic acids. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2022 Elektronická adresa https://academic.oup.com/nar/article/49/20/11425/6413599
Počet záznamů: 1