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Separation of regioisomers and enantiomers of triacylglycerols containing branched fatty acids (iso and/or anteiso)
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SYSNO ASEP 0546762 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Separation of regioisomers and enantiomers of triacylglycerols containing branched fatty acids (iso and/or anteiso) Tvůrce(i) Palyzová, Andrea (MBU-M) RID, ORCID
Cajthaml, Tomáš (MBU-M) RID, ORCID
Řezanka, Tomáš (MBU-M) ORCIDZdroj.dok. Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 42, č. 17-18 (2021), s. 1832-1843Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Branched fatty acids ; Enantiomeric separation ; Enzymatic hydrolysis ; rp-hplc/ms-esi+ ; Streptomyces ; Triacylglycerols Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000605174600001 EID SCOPUS 85099064420 DOI 10.1002/elps.202000320 Anotace A combination of two chromatographic and one enzymatic methods was used for identification of the molecular species of triacylglycerols (TAGs) from Streptomyces avermitilis. Streptomyces avermitliswas cultured on various carbon sources and the ratio of iso- (i-FAs), anteiso- (ai-FAs), and straight-chain- (n-FAs) fatty acids was modified by precursor-directed biosynthesis. Saturated TAGs were separated from other lipids (including TAGs containing unsaturated FAs) using Ag+ ion cartridges. Analysis of TAGs wereperformed by RP-HPLC/ESI+ tandem mass spectrometry. Both the synthetically prepared sn-TAGs and the natural mixture of TAGmolecular species of wereseparated and identified by tandem MS. The structures of synthetic TAGs werefurther confirmed by pancreatic lipase, which cleaves sn-TAGs into sn-2-monoacylglycerols. The retention times (tR) of the individual regioisomers and enantiomers were found to be depend on the structure of the TAGs. If one branched acyl (iso or anteiso) is present in the TAG molecule, then the elution order is enantiomer (n/n/br), opposite enantiomer (br/n/n), regioisomer (n/br/n). In the case where two branched acyls are in the TAG molecule, the order of the elution is different, that is, br/n/br, n/br/br, br/br/n. In all cases, it was further demonstrated that tandem MS of either synthetically prepared TAGs or TAGs obtained from natural material, that is, n-16:0/ai-15:0/n-16:0 and i-16:0/n-15:0/i-16:0 are identical. Unfortunately, it is not possible to distinguish by ESI+ tandem MS such TAGs, which differ only in the branching of the acyls. The results of our analyses of TAGs are in good agreement with previously published data in other streptomycetes. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202000320
Počet záznamů: 1