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Individual DNA Methylation Pattern Shifts in Nanoparticles-Exposed Workers Analyzed in Four Consecutive Years
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SYSNO ASEP 0545486 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Individual DNA Methylation Pattern Shifts in Nanoparticles-Exposed Workers Analyzed in Four Consecutive Years Tvůrce(i) Rössnerová, Andrea (UEM-P) RID
Hoňková, Kateřina (UEM-P)
Chvojková, Irena (UEM-P)
Pelcová, D. (CZ)
Ždímal, Vladimír (UCHP-M) RID, ORCID, SAI
Hubáček, J. A. (CZ)
Lischková, L. (CZ)
Vlčková, Š. (CZ)
Ondráček, Jakub (UCHP-M) RID, ORCID, SAI
Dvořáčková, Š. (CZ)
Topinka, Jan (UEM-P) RID, ORCID
Rössner ml., Pavel (UEM-P) RID, ORCIDČíslo článku 7834 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 22, č. 15 (2021)Poč.str. 19 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova DNA methylation ; epigenetics ; human ; time changes ; microarrays ; nanoparticles ; occupational exposure Vědní obor RIV DN - Vliv životního prostředí na zdraví Obor OECD Occupational health Vědní obor RIV – spolupráce Ústav chemických procesů - Vliv životního prostředí na zdraví CEP GA18-02079S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UEM-P - RVO:68378041 ; UCHP-M - RVO:67985858 UT WOS 000681888200001 EID SCOPUS 85110763922 DOI 10.3390/ijms22157834 Anotace A DNA methylation pattern represents an original plan of the function settings of individual cells and tissues. The basic strategies of its development and changes during the human lifetime are known, but the details related to its modification over the years on an individual basis have not yet been studied. Moreover, current evidence shows that environmental exposure could generate changes in DNA methylation settings and, subsequently, the function of genes. In this study, we analyzed the effect of chronic exposure to nanoparticles (NP) in occupationally exposed workers repeatedly sampled in four consecutive years (2016-2019). A detailed methylation pattern analysis of 14 persons (10 exposed and 4 controls) was performed on an individual basis. A microarray-based approach using chips, allowing the assessment of more than 850 K CpG loci, was used. Individual DNA methylation patterns were compared by principal component analysis (PCA). The results show the shift in DNA methylation patterns in individual years in all the exposed and control subjects. The overall range of differences varied between the years in individual persons. The differences between the first and last year of examination (a three-year time period) seem to be consistently greater in the NP-exposed subjects in comparison with the controls. The selected 14 most differently methylated cg loci were relatively stable in the chronically exposed subjects. In summary, the specific type of long-term exposure can contribute to the fixing of relevant epigenetic changes related to a specific environment as, e.g., NP inhalation. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/1422-0067/22/15/7834
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