A single-cell analytical approach to quantify activated caspase-3/7 during osteoblast proliferation, differentiation, and apoptosis

0543478 - ÚIACH 2022 RIV DE eng J - Článek v odborném periodiku
Killinger, Michael - Veselá, Barbora - Procházková, Markéta - Matalová, Eva - Klepárník, Karel
A single-cell analytical approach to quantify activated caspase-3/7 during osteoblast proliferation, differentiation, and apoptosis.
Analytical and Bioanalytical Chemistry. Roč. 413, č. 20 (2021), s. 5085-5093. ISSN 1618-2642. E-ISSN 1618-2650
Grant CEP: GA ČR(CZ) GA20-00726S
Institucionální podpora: RVO:68081715 ; RVO:67985904
Klíčová slova: apoptosis * differentation * single-cell analysis
Obor OECD: Analytical chemistry; Developmental biology (UZFG-Y)
Impakt faktor: 4.478, rok: 2021
Způsob publikování: Omezený přístup

The protein heterogeneity at the single-cell level has been recognized to be vital for an understanding of various life processes during animal development. In addition, the knowledge of accurate quantity of relevant proteins at cellular level is essential for
appropriate interpretation of diagnostic and therapeutic results. Here, we developed miniaturized instrumentation, and ultrasensitive and highly selective methodology based on bioluminescence photon-counting to quantify activated caspase-3/7 in individual cells.
Trvalý link: http://hdl.handle.net/11104/0320670