Počet záznamů: 1
Quantitative detection of optical anisotropy of single microtubules by polarization-sensitive interferometric scattering microscopy
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SYSNO ASEP 0543287 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Quantitative detection of optical anisotropy of single microtubules by polarization-sensitive interferometric scattering microscopy Tvůrce(i) Vala, Milan (URE-Y) RID
Palounek, David (URE-Y)
Robert, Marc Louis Hadrien (URE-Y)
Piliarik, Marek (URE-Y)Celkový počet autorů 4 Číslo článku 204001 Zdroj.dok. Journal of Physics D-Applied Physics. - : Institute of Physics Publishing - ISSN 0022-3727
Roč. 54, č. 20 (2021)Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova scattering anisotropy ; microtubule ; birefringence ; iSCAT ; tau protein Vědní obor RIV BH - Optika, masery a lasery Obor OECD Optics (including laser optics and quantum optics) CEP LL1602 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora URE-Y - RVO:67985882 UT WOS 000623879000001 DOI 10.1088/1361-6463/abe669 Anotace Microtubules (MTs) are ubiquitous cytoskeletal biopolymers essential for diverse cellular processes. MTs consist of strictly ordered tubulin dimers arranged into hollow cylindrical filaments and are known to be optically anisotropic, which enables their direct observation in microscopes based on polarization contrast. However, there are no experimental data to quantify the relation between the momentary optical anisotropy of the MT and the immediate arrangement of proteins in the MT structure. In this work, we introduce polarization-sensitive microscopy based on interferometric detection of scattering to quantify the scattering anisotropy of single unlabeled MTs with high precision. Our data explain the structural origin of MT anisotropy with a marginal contribution of the intrinsic asymmetry of a single tubulin molecule. We monitor changes in the MT scattering resulting from the binding of tau proteins to single MTs with a resolution of several proteins per diffraction-limited spot. We associate the changes in the contrast as well as in the scattering anisotropy with the formation of a shell around the MT formed by densely packed tau proteins. Our experimental results match closely with the theoretical model of the MT and include quantitative details about the polarization-dependent interaction of light with biological matter Pracoviště Ústav fotoniky a elektroniky Kontakt Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Rok sběru 2022 Elektronická adresa https://doi.org/10.1088/1361-6463/abe669
Počet záznamů: 1