Počet záznamů: 1
Quantitative detection of optical anisotropy of single microtubules by polarization-sensitive interferometric scattering microscopy
- 1.0543287 - ÚFE 2022 RIV GB eng J - Článek v odborném periodiku
Vala, Milan - Palounek, David - Robert, Marc Louis Hadrien - Piliarik, Marek
Quantitative detection of optical anisotropy of single microtubules by polarization-sensitive interferometric scattering microscopy.
Journal of Physics D-Applied Physics. Roč. 54, č. 20 (2021), č. článku 204001. ISSN 0022-3727. E-ISSN 1361-6463
Grant CEP: GA MŠMT(CZ) LL1602
Institucionální podpora: RVO:67985882
Klíčová slova: scattering anisotropy * microtubule * birefringence * iSCAT * tau protein
Obor OECD: Optics (including laser optics and quantum optics)
Impakt faktor: 3.409, rok: 2021
Způsob publikování: Open access
https://doi.org/10.1088/1361-6463/abe669
Microtubules (MTs) are ubiquitous cytoskeletal biopolymers essential for diverse cellular processes. MTs consist of strictly ordered tubulin dimers arranged into hollow cylindrical filaments and are known to be optically anisotropic, which enables their direct observation in microscopes based on polarization contrast. However, there are no experimental data to quantify the relation between the momentary optical anisotropy of the MT and the immediate arrangement of proteins in the MT structure. In this work, we introduce polarization-sensitive microscopy based on interferometric detection of scattering to quantify the scattering anisotropy of single unlabeled MTs with high precision. Our data explain the structural origin of MT anisotropy with a marginal contribution of the intrinsic asymmetry of a single tubulin molecule. We monitor changes in the MT scattering resulting from the binding of tau proteins to single MTs with a resolution of several proteins per diffraction-limited spot. We associate the changes in the contrast as well as in the scattering anisotropy with the formation of a shell around the MT formed by densely packed tau proteins. Our experimental results match closely with the theoretical model of the MT and include quantitative details about the polarization-dependent interaction of light with biological matter
Trvalý link: http://hdl.handle.net/11104/0320520
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