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Enzymatically cross-linked hydrogels based on synthetic poly(.alpha.-amino acid)s functionalized with RGD peptide for 3D mesenchymal stem cell culture
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SYSNO ASEP 0541734 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Enzymatically cross-linked hydrogels based on synthetic poly(.alpha.-amino acid)s functionalized with RGD peptide for 3D mesenchymal stem cell culture Tvůrce(i) Dvořáková, Jana (UMCH-V) RID, ORCID
Trousil, Jiří (UMCH-V) RID, ORCID
Podhorská, Bohumila (UMCH-V) ORCID
Mikšovská, Zuzana (UMCH-V)
Janoušková, Olga (UMCH-V) RID, SAI, ORCID
Proks, Vladimír (UMCH-V) RID, ORCIDZdroj.dok. Biomacromolecules. - : American Chemical Society - ISSN 1525-7797
Roč. 22, č. 4 (2021), s. 1417-1431Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova poly(α-amino acid) ; enzymatic cross-linking ; enzymatic degradation Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials CEP GA18-03224S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000640310700008 EID SCOPUS 85103370049 DOI 10.1021/acs.biomac.0c01641 Anotace Injectable hydrogel scaffolds combined with stem cell therapy represent a promising approach for minimally invasive surgical tissue repair. In this study, we developed and characterized a fully synthetic, biodegradable poly(N5-(2-hydroxyethyl)-l-glutamine)-based injectable hydrogel modified with integrin-binding arginine–glycine–aspartic acid (RGD) peptide (PHEG-Tyr-RGD). The biodegradable hydroxyphenyl polymer precursor derivative of PHEG-Tyr was enzymatically cross-linked to obtain injectable hydrogels with different physicochemical properties. The gelation time, gel yield, swelling behavior, and storage modulus of the PHEG-Tyr hydrogels were tuned by varying the concentrations of the PHEG-Tyr precursors and horseradish peroxidase as well as the nH2O2/nTyr ratio. The mechanical properties and gelation time of the PHEG-Tyr hydrogel were optimized for the encapsulation of rat mesenchymal stem cells (rMSCs). We focused on the 2D and 3D spreading and viability of rMSCs within the PHEG-Tyr-RGD hydrogels with different physicochemical microenvironments in vitro. Encapsulation of rMSCs shows long-term survival and exhibits cell–matrix and cell–cell interactions reflective of both the RGD concentration and hydrogel stiffness. The presented biomaterial represents a suitable biological microenvironment to guide 3D spreading and may act as a promising 3D artificial extracellular matrix for stem cell therapy. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2022 Elektronická adresa https://pubs.acs.org/doi/10.1021/acs.biomac.0c01641
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