Počet záznamů: 1
Endothelialization of an ePTFE vessel prosthesis modified with an antithrombogenic fibrin/heparin coating enriched with bound growth factors
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SYSNO ASEP 0539970 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Endothelialization of an ePTFE vessel prosthesis modified with an antithrombogenic fibrin/heparin coating enriched with bound growth factors Tvůrce(i) Táborská, Johanka (UMCH-V)
Riedelová, Zuzana (UMCH-V) ORCID
Brynda, Eduard (UMCH-V) RID
Májek, P. (CZ)
Riedel, Tomáš (UMCH-V) RID, ORCIDZdroj.dok. RSC Advances. - : Royal Society of Chemistry
Roč. 11, č. 11 (2021), s. 5903-5913Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova fibrin coating ; ePTFE vascular graft ; vascular endothelial growth factor Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Biomaterials (as related to medical implants, devices, sensors) CEP GA18-01163S GA ČR - Grantová agentura ČR LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000614616900006 EID SCOPUS 85100483467 DOI 10.1039/D1RA00053E Anotace Early and late thrombosis remain the most frequent reasons for the failure of synthetic cardiovascular grafts. Long-term hemocompatibility of implanted synthetic grafts can be achieved if a natural living endothelium is formed over its blood-contacting surface. Here we present a modification of a standard expanded polytetrafluorethylene (ePTFE) vessel prosthesis by a controlled preparation of a fibrin mesh enriched with covalently bound heparin and noncovalently bound vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF). Compared to a bare prosthesis, the coated prosthesis showed excellent antithrombogenic properties after contact with heparinized fresh human blood. Human umbilical vein endothelial cells seeded on the inner surface of the coated prosthesis formed a confluent layer in 5 days, whereas only small colonies of cells were scattered on the bare prosthesis. Viability of the cells was promoted mainly by FGF immobilized on the coating. These findings suggest that the coating may prevent acute thrombus formation and support the self-endothelialization of an implanted ePTFE vascular graft in vivo. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2022 Elektronická adresa https://pubs.rsc.org/en/content/articlelanding/2021/RA/D1RA00053E#!divAbstract
Počet záznamů: 1