Počet záznamů: 1
Determination of binding constants of 5-phenylazopyrimidines with β-cyclodextrin by affinity capillary electrophoresis
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SYSNO ASEP 0538657 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název Determination of binding constants of 5-phenylazopyrimidines with β-cyclodextrin by affinity capillary electrophoresis Tvůrce(i) Štěpánová, Sille (UOCHB-X) ORCID
Procházková, Eliška (UOCHB-X) RID, ORCID
Čechová, Lucie (UOCHB-X) RID, ORCID
Janeba, Zlatko (UOCHB-X) RID, ORCID
Kašička, Václav (UOCHB-X) RID, ORCIDZdroj.dok. Czech Chemical Society Symposium Series - ISSN 2336-7202
Roč. 18, č. 3 (2020), s. 83-84Poč.str. 2 s. Forma vydání Tištěná - P Akce Sjezd českých a slovenských chemických společností /72./ Datum konání 06.09.2020 - 09.09.2020 Místo konání Praha Země CZ - Česká republika Typ akce EUR Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova affinity capillary electrophoresis ; binding constant ; 5-phenylazopyrimidines Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GA18-02597S GA ČR - Grantová agentura ČR Institucionální podpora UOCHB-X - RVO:61388963 Anotace Non-covalent molecular interactions play important role in all living systems being essential to many chemical or biochemical processes. For characterization of these interactions, among other methods, also various modes of affinity capillary electrophoresis (ACE) have been employed. In this study, strength of non-covalent interactions of two 5 phenylazopyrimidines1 (see Fig. 1), with β-cyclodextrin (β CD) were estimated by mobility shift ACE (ms-ACE)2 . During ms-ACE measurements, 5-phenylazopyrimidines were dissolved in background electrolyte (BGE) free of β-CD, and injected as analytes. β-CD at various concentrations (0– 15 mM) was added to the BGE (18.5/42 mM Tris/H3PO4, pH 2.3). For 5-phenylazopyrimidines complexes with β-CD, 1:1 stoichiometry ratio was assumed. From the measured migration times, the effective mobilities of analytes were calculated and plotted against β-CD concentration in the BGE. For calculation of the apparent binding constant, a nonlinear regression analysis was applied. The apparent binding constants of complexes of compounds 1 and 2 with β-CD were estimated to be 22.9 ± 2.9 L/mol and 98.9 ± 3.6 L/mol, respectively. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2021
Počet záznamů: 1