Počet záznamů: 1
Histone Methyltransferase DOT1L Is Involved in Larval Molting and Second Stage Nymphal Feeding in Ornithodoros moubata
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SYSNO ASEP 0537402 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Histone Methyltransferase DOT1L Is Involved in Larval Molting and Second Stage Nymphal Feeding in Ornithodoros moubata Tvůrce(i) Gobl, Julia (BC-A) SAI
Sinha, Deepak Kumar (BC-A) RID, ORCID
Šíma, Radek (BC-A) RID, ORCID
Perner, Jan (BC-A) RID, ORCID
Kopáček, Petr (BC-A) RID, ORCID
Valdés, James J. (BC-A) RID, ORCID
Rego, Ryan O. M. (BC-A) RID, ORCID
Cabezas-Cruz, A. (FR)Celkový počet autorů 8 Číslo článku 157 Zdroj.dok. Vaccines. - : MDPI
Roč. 8, č. 2 (2020)Poč.str. 14 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova rhipicephalus boophilus microplus ; tick ; methylation ; acari ; chromatin ; midgut ; domain ; genes ; Ornithodoros moubata ; histone methyltransferase ; dot1l Vědní obor RIV EC - Imunologie Obor OECD Immunology CEP GA18-01832S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000550820800001 EID SCOPUS 85083217982 DOI 10.3390/vaccines8020157 Anotace Epigenetic mechanisms have not been characterized in ticks despite their importance as vectors of human and animal diseases worldwide. Our investigation identifies and functionally characterizes the orthologue of S-adenosylmethionine (SAM) binding methyltransferase enzyme, disruptor of telomeric silencing 1-like (DOT1L) in Ornithodoros moubata (OmDOT1L), a soft tick vector for the relapsing fever pathogen Borrelia duttonii and the African swine fever virus. The OmDOT1L tertiary structure was predicted and compared to the Homo sapiens DOT1L which had been co-crystalized with SGC0946, a DOT1L-specific inhibitor. The amino acid residues crucial for SAM and SGC0946 binding conserved in most DOT1L sequences available, are also conserved in OmDOT1L. Quantitative PCR of Omdot1l during O. moubata life stages showed that transcripts were significantly upregulated in first-stage nymphs. O. moubata larvae exposed to SGC0946 displayed high mortality during molting to first-stage nymphs. Furthermore, a significant decrease in weight was observed in second-stage nymphs fed on recombinant OmDOT1L-immunized rabbits. In contrast, artificial blood feeding supplemented with SGC0946 did not affect survival and reproductive performance of adult female ticks. We concluded that OmDOT1L plays an essential role in the regulation of larval molting and the feeding of O. moubata second-stage nymphs. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2021 Elektronická adresa https://www.mdpi.com/2076-393X/8/2/157
Počet záznamů: 1