Počet záznamů: 1
Programmed Cell Death in the Left and Right Ventricle of the Late Phase of Post-Infarction Heart Failure
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SYSNO ASEP 0535047 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Programmed Cell Death in the Left and Right Ventricle of the Late Phase of Post-Infarction Heart Failure Tvůrce(i) Lichý, M. (SK)
Szobi, A. (SK)
Hrdlička, Jaroslav (FGU-C) ORCID, RID
Neckář, Jan (FGU-C) RID, ORCID
Kolář, František (FGU-C) RID, ORCID, SAI
Adameová, A. (SK)Číslo článku 7782 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 21, č. 20 (2020)Poč.str. 18 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova heart failure ; cell death ; autophagy ; necroptosis Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP NV15-27735A GA MZd - Ministerstvo zdravotnictví Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000585707400001 EID SCOPUS 85094117044 DOI 10.3390/ijms21207782 Anotace While necroptosis has been shown to contribute to the pathogenesis of post-infarction heart failure (HF), the role of autophagy remains unclear. Likewise, linkage between these two cell death modalities has not been sufficiently investigated. HF was induced by 60-min left coronary occlusion in adult Wistar rats and heart function was assessed 6 weeks later followed by immunoblotting analysis of necroptotic and autophagic proteins in both the left (LV) and right ventricle (RV). HF had no effect on RIP1 and RIP3 expression. PhosphoSer229-RIP3, acting as a pro-necroptotic signal, was increased in LV while deceased in RV of failing hearts. Total MLKL was elevated in RV only. Decrease in pSer555-ULK1, increase in pSer473-Akt and no significant elevation in beclin-1 and LC3-II/I ratio indicated rather a lowered rate of autophagy in LV. No beclin-1 upregulation and decreased LC3 processing also suggested the inhibition of both autophagosome formation and maturation in RV of failing hearts. In contrast, p89 PARP1 fragment, a marker of executed apoptosis, was increased in RV only. This is the first study showing a different signaling in ventricles of the late phase of post-infarction HF, highlighting necroptosis itself rather than its linkage with autophagy in LV, and apoptosis in RV. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2021 Elektronická adresa https://www.mdpi.com/1422-0067/21/20/7782
Počet záznamů: 1