Počet záznamů: 1
Exosomal transfer of miR-126 promotes the anti-tumour response in malignant mesothelioma: Role of miR-126 in cancer-stroma communication
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SYSNO ASEP 0521316 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Exosomal transfer of miR-126 promotes the anti-tumour response in malignant mesothelioma: Role of miR-126 in cancer-stroma communication Tvůrce(i) Monaco, F. (IT)
Gaetani, S. (IT)
Alessandrini, F. (IT)
Tagliabracci, A. (IT)
Bracci, M. (IT)
Valentino, M. (IT)
Neužil, Jiří (BTO-N) RID
Amati, M. (IT)
Bovenzi, M. (IT)
Tomasetti, M. (IT)
Santarelli, L. (IT)Celkový počet autorů 11 Zdroj.dok. Cancer letters. - : Elsevier - ISSN 0304-3835
Roč. 463, č. 2019 (2019), s. 27-36Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova pleural mesothelioma ; angiogenesis ; cells ; repression ; apoptosis Vědní obor RIV FD - Onkologie a hematologie Obor OECD Oncology CEP NV16-31604A GA MZd - Ministerstvo zdravotnictví Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 UT WOS 000486135000003 DOI 10.1016/j.canlet.2019.08.001 Anotace MiR-126 has been shown to suppress malignant mesothelioma (MM) by targeting cancer-related genes without inducing toxicity or histopathological changes. Exosomes provide the opportunity to deliver therapeutic cargo to cancer stroma. Here, a tumour stromal model composed of endothelial cells (HUVECs), fibroblasts (IMR-90 cells), non-malignant mesothelial cells (Met-5A cells) and MM cells (H28 and MM-B1 cells) was used. The cells were treated with exosomes from HUVECs carrying endogenous (exo-HUVEC) and enriched miR-126 (exo-HUVECmiR-126), and the uptake/turnover of exosomes, miR-126 distribution within the stroma, and effect of miR-126 on cell signalling, angiogenesis and cell proliferation were evaluated. Based on the sensitivity of MM cells to exo-HUVEC miR-126 treatment, miR-126 was distributed differently across stromal cells. The reduced miR-126 content in fibroblasts in favour of endothelial cells reduced angiogenesis and suppressed cell growth in an miR-126-sensitive environment. Conversely, the accumulation of miR-126 in fibroblasts and the reduced level of miR-126 in endothelial cells induced tube formation in an miR-126-resistant environment via VEGF/EGFL7 upregulation and IRS1-mediated cell proliferation. These findings suggest that transfer of miR-126 via HUVEC-derived exosomes represents a novel strategy to inhibit angiogenesis and cell growth in MM. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2020 Elektronická adresa https://www.sciencedirect.com/science/article/abs/pii/S0304383519304240?via%3Dihub
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