Počet záznamů: 1
Mechanisms of sublethal copper toxicity damage to the photosynthetic apparatus of Rhodospirillum rubrum
- 1.
SYSNO ASEP 0511086 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Mechanisms of sublethal copper toxicity damage to the photosynthetic apparatus of Rhodospirillum rubrum Tvůrce(i) Jaime-Perez, Noelia (BC-A)
Kaftan, David (MBU-M) ORCID
Bína, David (BC-A) RID, ORCID
Bokhari, Syed Nadeem Hussain (BC-A) RID
Shreedhar, Sowmya (BC-A)
Küpper, Hendrik (BC-A) RID, ORCIDCelkový počet autorů 6 Zdroj.dok. Biochimica Et Biophysica Acta-Bioenergetics. - : Elsevier - ISSN 0005-2728
Roč. 1860, č. 8 (2019), s. 640-650Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Bacteriochlorophyll ; Purple bacteria ; Copper toxicity ; Light harvesting complex ; Photosynthesis Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology Vědní obor RIV – spolupráce Mikrobiologický ústav - Biochemie CEP EF15_003/0000336 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA17-14024S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 ; MBU-M - RVO:61388971 UT WOS 000480668900005 EID SCOPUS 85068623556 DOI 10.1016/j.bbabio.2019.06.004 Anotace Magnesium (Mg2+) is the ubiquitous metal ion present in chlorophyll and bacteriochlorophyll (BChl), involved in photosystems in photosynthetic organisms. In the present study we investigated targets of toxic copper binding to the photosynthetic apparatus of the anoxygenic purple bacterium Rhodospirillum rubrum. This was done by a combination of in vivo measurements of flash photolysis and fast fluorescence kinetics combined with the analysis of metal binding to pigments and pigment-protein complexes isolated from Cu-stressed cells by HPLC-ICPMS (ICP-sfMS). This work concludes that R. rubrum is highly sensitive to Cu2+, with a strong inhibition of the photosynthetic reaction centres (RCs) already at 2 mu M Cu2+. The inhibition of growth and of RC activity was related to the formation of Cu-containing BChl degradation products that occurred much more in the RC than in LH1. These results suggest that the shift of metal centres in BChl from Mg2+ to Cu2+ can occur in vivo in the RCs of R. rubrum under environmentally realistic Cu2+ concentrations, leading to a strong inhibition of the function of these RCs. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2020 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0005272819300568?via%3Dihub
Počet záznamů: 1