Počet záznamů: 1
Distinguishing the glycan isomers 2,3-sialyllactose and 2,6-sialyllactose by voltammetry after modification with osmium(VI) complexes
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SYSNO ASEP 0507731 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Distinguishing the glycan isomers 2,3-sialyllactose and 2,6-sialyllactose by voltammetry after modification with osmium(VI) complexes Tvůrce(i) Trefulka, Mojmír (BFU-R) RID, ORCID
Černocká, Hana (BFU-R) RID, ORCID
Fojt, Lukáš (BFU-R) RID, ORCID
Paleček, Emil (BFU-R) RID, ORCID
Ostatná, Veronika (BFU-R) RID, ORCIDCelkový počet autorů 5 Zdroj.dok. Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670
Roč. 1067, AUG 27 2019 (2019), s. 56-62Poč.str. 7 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova glycosylation ; strategies ; proteins ; oligosaccharides ; polysaccharides Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GA18-18154S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora BFU-R - RVO:68081707 UT WOS 000466150300005 DOI 10.1016/j.aca.2019.03.060 Anotace Altered glycosylation is a universal feature of cancer cells and certain glycans are well-known markers of tumor progression. In this work we studied two glycan isomers, 2,3-sialyllactose (3-SL) and 2,6-sialyllactose (6-SL), frequently appearing in glycoproteins connected with cancer. A combination of square wave voltammetry and glycan modification with osmium(VI) N, N, N', N'-tetramethylethylenedi-amine (Os(VI) tem) allowed to distinguish between these regioisomers, since the 6-SL molecule can bind three Os(VI), while the 3-SL only two Os(VI) moieties, as experiments using capillary electrophoresis, inductively coupled plasma mass spectrometry and thin layer chromatography showed. A similar pattern of Os(VI)-modification was found for isomers of sialyl-N-acetyllactosamine and sialylgalactose. Covalent adducts of Os(VI) tem with glycans yielded three reduction voltammetric peaks. The ratio of peak I/peak II heights depends on the content of individual regioisomer in the sample. Our proposed approach allows the determination of isomer percentage representation in the mixture after one voltammogram recording. These results show a new appropriate method for the discrimination of glycan isomers containing terminal sialic acid important for distinguishing between cancerous and non-cancerous origin of biomarkers. (C) 2019 Elsevier B.V. All rights reserved. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2020 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0003267019303782?via%3Dihub
Počet záznamů: 1