Počet záznamů: 1
Nanostructures in Biomedicine: Their Regenerative and toxic properties
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SYSNO ASEP 0500086 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Hepatocyte Growth on Smooth and Nanoporous Polymer Nanofiber Sheets Tvůrce(i) Mareková, Dana (UEM-P)
Jendelová, Pavla (UEM-P) RID, ORCIDZdroj.dok. Nanostructures in Biomedicine: Their Regenerative and toxic properties. - Praha 3 : Proxima Tisk, s.r.o., 2018 / Pamula Elzbieta - ISBN 978-80-906655-7-6 Rozsah stran s. 129-136 Poč.str. 8 s. Poč.str.knihy 198 Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova nanofibers ; masenchymal stem cells ; hepatocytes Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biophysics CEP LO1309 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEM-P - RVO:68378041 Anotace Nanofibers are an attractive material, in which we can match their mechanical and chemical properties. Liver plays a major role in detoxification, protein synthesis, glycogen storage and many other functions. They form a complex structure composed from hepatocytes (60%), endothelial cells (10-20%), Kupfer cells (15-25%) and others. Mesenchymal stem cells (MSC) are multipotent stem cells isolated from the bone morrow and other tissues. They have significant immunomodulatory and trophic properties. Therefore we used MSC for co-cultivation with hepatocytes. In this study we created scaffolds for tissue engineering utilizing the methacrylate copolymer (HEMA-EOEMA) and poly-α-caprolactone (PCL) nanofibers as artificial extracellular matrix seeded with hepatocytes. We have assessed their viability and albumin production as an index of liver function. Moreover, the secretion of aspartate transaminase (AST) and alkaline liver phosphatase (ALP) have been measured in order to determine hepatocyte stress. In addition, co-cultures of hepatocytes and BMSC were maintained on nanofiber layers to evaluate the ability of MSC to support hepatocytes on nanofibers. The results show that the best carrier for cultivation of hepatocytes is PCL/EtAc/DMSO. Co-cultures of hepatocytes and BMSC increased the production of albumin and decreased secretion of stress markers within the time. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2019
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